endo-α-N-Acetylgalactosaminidase (=Endo-α) Recombinant: from Bifidobacterium longum expressed in Escherichia coli

×

纯度/分析方法:

别名：

• 内-α-N-乙酰半乳糖氨酶 (=内-α) 重组:在大肠杆菌中表达的长双歧杆菌属
• Endo-α Recombinant: from Bifidobacterium longum expressed in Escherichia coli

产品文档：

SDS | 技术规格 | CoA及其他文档 | 分析图谱

基本信息 | 技术规格和文件 | 安全和法规 | 应用实例・文献

补充产品信息

产品详情页面说明

规格	单价	同一天	2-3个工作日	左侧以上货期	数量	货期信息
1VIAL	S$273.00	9	0	请联系我们		货期预估

* 订购TCI产品，请联系我们的经销商或 联系我们。
* TCI会时常优化储存条件，敬请留意。

添加到您的购物车

添加到购物车

大包装询价

Product Information

• Quantity : 100 mU per vial
• Product Form : Purified Endo-α, Lyophilized powder
• Preservative : None
• Stabilizer : None

Description

• This Endo-α is a glycoside hydrolase that recognizes the structure of the Galβ1-3GalNAc disaccharide α-linked with a hydroxyl group and it releases Galβ1-3GalNAc (galacto-N-biose) by hydrolysis. The Endo-α gene is derived from Bifidobacterium longum, and its recombinant protein is expressed in Escherichia coli.

Unit Definition

• One unit (U) of the Endo-α is defined as the amount required to produce 1 μmol of Galβ1-3GalNAc from Galβ1-3GalNAc α-pNP (Product No. G0375) per minute at 37°C under the condition of pH 5.0.

Assay Conditions

• Reaction conditions: 50 mM Sodium acetate, pH 5.0
  The reaction mixture includes the enzyme and Galβ1-3GalNAc α-pNP in 20 μL. The mixture is incubated at 37°C for 10 minutes. After the enzyme reaction, quenching is performed by adding 30 μL of 0.25 M sodium carbonate. Quantification of released pNP is measured by a UV spectrometer.

Storage

• Store at -80°C in its lyophilized powder form. Use promptly after dissolving in buffer.

Related Products

• I1205 Sialidase (Neuraminidase)
• I1206 Sialidase (Neuraminidase) Agarose
• G0622 O-Glycosidase for core1 (Endo-α and Sialidase mix)
• 
  
• Other oligosaccharide-releasing enzymes
• [Endo-type N-glycanase (ENGase)]
• A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
• E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
• G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
• 
  
• [Endo-type O-glycanase]
• A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
• 
  
• [Endo-type glycoceramidase]
• R0240 rEGCase I
• R0241 rEGCase I assisted by Activator II
• R0242 rEGCase II
• R0243 rEGCase II assisted by Activator II
• 
  
• [Endo-type glycosaminoglycanase]
• K0069 Keratanase II (for hydrolysis of Keratan sulfate)