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CAS RN: 254973-02-9 | 产品编码: A5576
AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]
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![AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]")
| 产品编码 | A5576 |
| 纯度/分析方法 | >95.0%(HPLC) |
| 分子式/分子量 | C__8H__7N__3O__2S = 209.22 |
| 外观与形状（20°C） | 固体 |
| 储存温度 | 冷藏 (0-10°C) |
| 储存在惰性气体下 | 存放于惰性气体之中 |
| 应避免的情况 | 空气,加热 |
| 包装和容器 | 100MG-Glass Bottle with Plastic Insert （查看图片） |
| CAS RN | 254973-02-9 |
| PubChem物质ID | 87563193 |
| MDL编号 | MFCD04038408 |
技术规格
| Appearance | Light yellow to Brown powder to crystal |
| Purity(HPLC) | min. 95.0 area% |
| Effect test of HPLC derivatization | Effective as labeling agent for Caproic Acid, Lauric Acid, Myristic Acid, Palmitic Acid |
物性（参考值）
GHS
相关法规
运输信息
| HS编码* | 2934.99-000 |
应用
HPLC Labeling Reagent for Carboxylic Acids
The compound 1, an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a mercapto group, easily reacts with a carboxyl group to form the corresponding thioester. 1 itself fluoresces very little, but the thioester derivatives fluoresce highly. The resultant thioester is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 368 nm and 524 nm, respectively. [The detection limit = 10~20 fmol (S/N = 3)]
Application example:
Add 20 µL of mixed fatty acid / acetonitrile solution, 20 µL of 20 mM labeling reagent 1 / dichloromethane solution, 20 µL of triphenylphosphine / acetonitrile solution and 20 µL of 2,2’-dipyridyl disulfide / acetonitrile solution to a 500 µL vessel, and the mixture is left at room temperature for 15 min. Dilute this reactant mixture with 20 µL of acetonitrile, and use 1 µL of this diluted solution as an HPLC sample solution.
Add 20 µL of mixed fatty acid / acetonitrile solution, 20 µL of 20 mM labeling reagent 1 / dichloromethane solution, 20 µL of triphenylphosphine / acetonitrile solution and 20 µL of 2,2’-dipyridyl disulfide / acetonitrile solution to a 500 µL vessel, and the mixture is left at room temperature for 15 min. Dilute this reactant mixture with 20 µL of acetonitrile, and use 1 µL of this diluted solution as an HPLC sample solution.
TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )
HPLC Labeling Reagents ( PDF 10MB )
References
参考文献
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