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CAS RN: | 产品编码: B7077
Biotin Quantitation Kit
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Product Information
- Components : Avidin Buffer 6.25 mL, Biotin Standard (in DMSO) (10mM Biotin in DMSO) 0.1 mL, HABA Buffer 25 mL
- Size : Approx. 34 tests (Cuvette). 170 tests (96-well plate)
Description
- Avidin-biotin binding, the most robust non-covalent protein-ligand interaction in nature, is widely used in biotechnology. When HABA binds to avidin, the resulting complex absorbs light at 500 nm. When a sample containing biotin is added, the HABA is displaced by biotin binding to the avidin. Consequently, the absorbance at 500 nm decreases proportionally to the amount of biotin bound to HABA.
Storage
- Store at 4 ºC. Use within 1 year.
Important Information
- After biotin labeling, remove any unreacted biotinylation reagent from the sample that will be used for biotin quantification using a column or dialysis.
The sample must be dissolved in PBS or TBS for biotin quantification. Avoid buffers containing potassium. If you wish to use a different buffer, compare it with PBS or TBS to ensure there are no issues.
Step-by-Step Protocol
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| 产品编码 | B7077 |
| 外观与形状（20°C） | 液体 |
储存温度 
|
冷藏 (0-10°C) |
| 应避免的情况 | 加热 |
技术规格
物性（参考值）
GHS
| 信号词 | Warning |
| 危险性说明 | H227 : Combustible liquid. |
| 防范说明 | P501 : Dispose of contents/ container to an approved waste disposal plant. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P280 : Wear protective gloves/ eye protection/ face protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P403 : Store in a well-ventilated place. |
相关法规
运输信息
| HS编码* | 3822.19-000 |
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