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CAS RN: 1620086-77-2 | 产品编码: C3326
5-Chlorosaligenyl-N,N-diisopropylphosphoramidite
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| 产品编码 | C3326 |
纯度/分析方法 
|
>95.0%(T) |
| 分子式/分子量 | C__1__3H__1__9ClNO__2P = 287.72 |
| 外观与形状（20°C） | 固体 |
| 储存温度
|
冷藏 (0-10°C) |
| 储存在惰性气体下 | 存放于惰性气体之中 |
| 应避免的情况 | 空气,湿气 (分解),加热 |
| 包装和容器
|
1G-Glass Bottle with Plastic Insert （查看图片）, 200MG-Glass Bottle with Plastic Insert （查看图片） |
| CAS RN | 1620086-77-2 |
| Reaxys-RN | 28732172 |
| PubChem物质ID | 354334377 |
技术规格
| Appearance | White to Almost white powder to lump |
| Purity(Nonaqueous Titration) | min. 95.0 % |
| NMR | confirm to structure |
物性（参考值）
| 熔点 | 45 °C |
GHS
| 象形图 |
|
| 信号词 | Warning |
| 危险性说明 | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
| 防范说明 | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
相关法规
运输信息
| HS编码* | 2934.99-000 |
应用
Solid-phase Synthesis of DNA and RNA 5'-O-Triphosphates
After oligonucleotide synthesis:
5-Chloro-saligenyl-N,N-diisopropylphosphoramidite (Reagent I, C3326) is coupled to the 5'-end of the DMT-off oligonucleotide. Oxidation of the formed 5'-phosphite using the same oxidizing reagent used in standard oligonucleotide synthesis leads to support-bonded 5'-cycloSal-oligonucleotides. Reaction with pyrophosphate (Reagent II, B5440) yields the corresponding 5'-triphosphates. The 5'-triphosphorylated oligonucleotides are obtained after cleavage from the support in high purity and excellent yields. The whole reaction sequence was adapted to be used on a standard oligonucleotide synthesizer.
5-Chloro-saligenyl-N,N-diisopropylphosphoramidite (Reagent I, C3326) is coupled to the 5'-end of the DMT-off oligonucleotide. Oxidation of the formed 5'-phosphite using the same oxidizing reagent used in standard oligonucleotide synthesis leads to support-bonded 5'-cycloSal-oligonucleotides. Reaction with pyrophosphate (Reagent II, B5440) yields the corresponding 5'-triphosphates. The 5'-triphosphorylated oligonucleotides are obtained after cleavage from the support in high purity and excellent yields. The whole reaction sequence was adapted to be used on a standard oligonucleotide synthesizer.
References
- Solid-Phase Synthesis of DNA and RNA 5’-O-Triphosphates Using cycloSal Chemistry
PubMed Literature
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