Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]

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纯度/分析方法:

别名：

• 化学发光HRP底物溶液试剂盒 [用于蛋白免疫印迹]

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规格	单价	同一天	2-3个工作日	左侧以上货期	数量	货期信息
1KIT	S$232.50	≥60	0	请联系我们		货期预估

* 订购TCI产品，请联系我们的经销商或 联系我们。
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Product Information

• Components : Chemiluminescence HRP Substrate Solution A 100 mL×1, Chemiluminescence HRP Substrate Solution B 100 mL×1
• Volume : 1 kit (for 2000 cm² of membrane)

Description

• This product is a reagent used for the chemiluminescent detection of membrane-transferred HRP-labeled proteins in Western blotting. Immediately before use, solutions A and B are mixed 1:1 and applied to the membrane. Proteins can be detected down to nanogram level.

Storage

• The product should be stored at 4 °C and protected from light.

Direction for Use

• 1. Bring Solution A and Solution B to room temperature.
  2. Prepare a western blot membrane labeled with an HRP-conjugated probe.
  3. Wash the membrane three times for 10 minutes each time with 1× TBS-T buffer.
  4. Mix Solution A and Solution B in a 1:1 ratio immediately before use. The recommended volume is 0.1 mL/cm² membrane.
  5. Place the membrane protein side up on a sheet of the plastic wrap or polyethylene sheet.
  6. Gently pour the mixture evenly over the surface of the membrane.
  7. Incubate for 60 seconds at room temperature.
  8. Remove excess reagent by blotting with a tissue or tilting the membrane upright to allow excess to run off.
  9. 9. Place the membrane on a clean sheet of plastic wrap or polyethylene.
  10. Image the membrane using an imaging system or X-ray film.

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