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CAS RN: 31282-04-9 | 产品编码: H1509
Hygromycin B
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| 产品编码 | H1509 |
纯度/分析方法 
|
>80.0%(HPLC) |
| 分子式/分子量 | C__2__0H__3__7N__3O__1__3 = 527.52 |
| 外观与形状（20°C） | 固体 |
| 储存温度
|
冷藏 (0-10°C) |
| 应避免的情况 | 加热 |
| 包装和容器
|
1G-Glass Bottle with Plastic Insert （查看图片）, 200MG-Glass Bottle with Plastic Insert （查看图片） |
| CAS RN | 31282-04-9 |
| Reaxys-RN | 66457 |
| PubChem物质ID | 253661073 |
| Merck Index (14) | 4852 |
| MDL编号 | MFCD06795479 |
技术规格
| Appearance | White to Light yellow to Light orange powder to crystal |
| Purity(HPLC)(CAD) | min. 80.0 area% |
| Specific rotation [a]20/D | +18.0 to +23.0 deg(C=1, H2O) |
| NMR | confirm to structure |
物性（参考值）
| 熔点 | 180 °C(dec.) |
| 比旋光度 [α]D | 20° (C=1,H2O) |
GHS
| 象形图 |
|
| 信号词 | Danger |
| 危险性说明 | H300 + H310 + H330 : Fatal if swallowed, in contact with skin or if inhaled. H318 : Causes serious eye damage. H317 : May cause an allergic skin reaction. H334 : May cause allergy or asthma symptoms or breathing difficulties if inhaled. |
| 防范说明 | P501 : Dispose of contents/ container to an approved waste disposal plant. P272 : Contaminated work clothing should not be allowed out of the workplace. P260 : Do not breathe dust/ fume/ gas/ mist/ vapors/ spray. P270 : Do not eat, drink or smoke when using this product. P262 : Do not get in eyes, on skin, or on clothing. P271 : Use only outdoors or in a well-ventilated area. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection. P284 : Wear respiratory protection. P342 + P311 : If experiencing respiratory symptoms: Call a POISON CENTER/doctor. P361 + P364 : Take off immediately all contaminated clothing and wash it before reuse. P333 + P313 : If skin irritation or rash occurs: Get medical advice/ attention. P301 + P310 + P330 : IF SWALLOWED: Immediately call a POISON CENTER/doctor. Rinse mouth. P302 + P352 + P310 : IF ON SKIN: Wash with plenty of water. Immediately call a POISON CENTER/doctor. P304 + P340 + P310 : IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor. P305 + P351 + P338 + P310 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor. P403 + P233 : Store in a well-ventilated place. Keep container tightly closed. P405 : Store locked up. |
相关法规
| RTECS# | WK2130000 |
运输信息
| UN编号 | UN3462 |
| 类别 | 6.1 |
| 包装类别 | I |
| HS编码* | 2941.90-000 |
应用
Hygromycin B: A Selective Reagent in Molecular Genetics Experiments on Cells
Hygromycin B is a water-soluble aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus. It is acts by binding to the 80S subunit of the bacterial ribosome and inhibiting protein synthesis. In molecular genetics, hygromycin B is used in genetic modification as a selective reagent to isolate prokaryotic and eukaryotic cells coupled to a gene coding for hygromycin resistance.
References
- Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae
- Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells
- Resistance to hygromycin B. A new marker for plant transformation studies
- Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli
- Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene
- Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method
参考文献
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