text.skipToContent text.skipToNavigation

Maximum quantity allowed is 999

请选择数量

CAS RN: | 产品编码: H1621

Horseradish Peroxidase Maleimide Conjugate (0.5mg×3)


纯度/分析方法:
别名:
  • 辣根过氧化物酶马来酰亚胺结合物 (0.5mg×3)
  • HRP马来酰亚胺 (0.5mg×3)
  • 辣根过氧化物酶马来酰亚胺活化物 (0.5mg×3)
  • HRP Maleimide (0.5mg×3)
  • Horseradish Peroxidase Maleimide Activated (0.5mg×3)
产品文档:
1SET
S$192.00
34   6   请联系我们

* 订购TCI产品,请联系我们的经销商或 联系我们。
* TCI会时常优化储存条件,敬请留意。


补充产品信息:

Product Information

Volume/Quantity : 0.5 mg x 3 vial
Conjugation : Peroxidase from Horseradish (HRP) was conjugated to Sulfo-SMCC (Product No. S0883).
Product Form : Horseradish Peroxidase Maleimide Conjugate. Lyophilized in 10 mM sodium phosphate, 0.115 mM NaCl, 40 mM sucrose, pH 6.6.
Preservative : None

Description

HRP Maleimide Conjugate is designed to facilitate the conjugation of the HRP to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups. Activated HRP contains 1–3 maleimide groups for each HRP molecule.


Protocol

  1. Firstly return the vial to room temperature.
  2. Reconstitute the contents of the vial with 100μL of buffer or water to obtain a 5 mg/mL solution of HRP Maleimide.
    Note: Do not vortex or heat the HRP Maleimide.
  3. Dissolve the sulfhydryl compound to be conjugated at 2-5 mg/ml in the buffer.
    Note: We recommend that the thiol-containing molecule is dissolved in the pH range 6.5-7.5 buffer. 0.1M sodium phosphate, 0.15M NaCl, 10mM EDTA at pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA may help to prevent metal catalysed oxidation of thiols. Use of a 4:1 (HRP Maleimide:antibody) molar ratio in the conjugation reaction usually results in high-activity conjugates. This vial contains 0.5 mg of HRP Maleimide Conjugate and can effectively modify 0.5 mg of immunoglobulin.
  4. Immediately mix the thiol-containing molecule and HRP Maleimide solutions and allow them to react for 2 hours at room temperature. To increase HRP incorporation, extend reaction time up to 12 hours.
  5. If desired, desalt, dialyze or purify the conjugate.

Storage

Store at −20 °C to −80 °C. Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.


Related Products

A0296 2-Aminoethanethiol Hydrochloride
B5944 Bovine Serum Albumin Maleimide Conjugate (1mg×3)
D3647 DL-Dithiothreitol [for Electrophoresis]
T3531 Streptavidin Maleimide Conjugate
S0431 N-Succinimidyl S-Acetylthioglycolate [Cross-linking Reagent]
S0859 N-Succinimidyl 3-(Acetylthio)propionate
H1746 Horseradish Peroxidase NHS Ester Conjugate (0.2mg×3)
A3294 Anti-NeuGc Polyclonal Antibody Biotin Conjugate
G0387 Goat Anti-Mouse IgG Biotin Conjugate
G0389 Goat Anti-Rabbit IgG Biotin Conjugate
G0432 Goat Anti-Mouse IgM Biotin Conjugate
G0569 Goat Anti-Mouse IgG R-PE Conjugate
G0577 Goat Anti-Rabbit IgG R-PE Conjugate
H1619 Sheep Anti-Chicken IgY Biotin Conjugate
S0951 Streptavidin from Streptomyces avidinii
S0966 Streptavidin FITC Conjugate
S0972 Streptavidin HRP Conjugate
S0993 Streptavidin DTBTA-Eu3+ Conjugate
T3531 Streptavidin Maleimide Conjugate

产品编码 H1621
外观与形状(20°C) 固体
储存温度 冷冻 (-20°C)
应避免的情况 加热
技术规格
Appearance Light yellow to Brown powder to crystal
Molar Ratio Maleimido 1-3 moles of maleimide per mole of HRP
物性(参考值)
GHS
相关法规
运输信息
HS编码* 3507.90-000
*此H.S.编码用于日本出口报关,不适用于您所在国家或地区的进口申报
产品文档 (部分产品的分析图谱无法提供,敬请谅解。)
化学品安全说明书(SDS)
请选择语言。

如需更多帮助,请联系我 们。

技术规格
CoA及其他文档
请输入批号 批号输入有误。请输入中横线前的4-5个字母数字字符。

所搜索批号的产品已停产,无相关文档。

示例 CoA
可下载CoA示例。注:该示例不一定是最新批次的CoA。

目前没有该产品的 CoA 示例。

分析图谱
请输入批号 批号输入有误。请输入中横线前的4-5个字母数字字符。

很抱歉,您搜索的分析图谱无法提供。

所搜索批号的产品已停产,无相关文档。

其他文件

会话状态
当前会话将在10分钟后超时,并返回主页。请点击按钮继续浏览。分钟后超时,并返回主页。请点击按钮继续浏览。

您的会话已超时,将返回至主页。