Silver Stain Kit [for Electrophoresis]

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纯度/分析方法:

别名：

• 银染色试剂盒 [电泳用]

产品文档：

SDS | 技术规格 | CoA及其他文档 | 分析图谱

基本信息 | 技术规格和文件 | 安全和法规 | 应用实例・文献

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产品详情页面说明

规格	单价	同一天	2-3个工作日	左侧以上货期	数量	货期信息
1KIT	S$219.00	16	0	请联系我们		货期预估

* 订购TCI产品，请联系我们的经销商或 联系我们。
* TCI会时常优化储存条件，敬请留意。

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大包装询价

Product Information

• Components : Solution-1 (Enhancing solution) 50 mL, Solution-2 (Staining solution) 50 mL, Solution-3 (Developer solution #1) 50 mL, Solution-4 (Developer solution #2) 50 mL
• Size : 50 tests (90 mm x 80 mm, 1 mm gel)

Description

• Silver staining is a highly-sensitive and rapid detection method for SDS-PAGE separated proteins or nucleic acids. It is several times more sensitive than the commonly used EtBr or CBB staining methods.

Direction for Use

[Solution Preparation]

• Agitate each included solution well, and prepare the following 4 solutions in advance.
  The volumes of reagents given below is based on a gel size of 90 mm x 80 mm x 1 mm. For different sizes of gel, the volume of reagents should be adjusted accordingly. For methanol and acetic acid, it is recommended to use special grade or any other higher grade.
• Fixing Solution : Deionized water : 40 mL + Methanol : 50 mL + Acetic acid : 10 mL + Solution-1 (Enhancing solution) : 1 mL
• Staining Solution : Deionized water : 100 mL + Solution-2 (Staining solution) : 1 mL
• Developer Solution : Deionized water : 200 mL + Solution-3 (Developer solution #1) : 1 mL + Solution-4 (Developer solution #2) : 1 mL
• Stop Solution : Deionized water : 100 mL + Acetic acid : 1 mL

[Usage]

1. Soak the gel in 100 mL of Fixing Solution in a clean tray and incubate it with shaking for 10 minutes.
2. Remove the solution from step 1, and wash gel with 100 mL of deionized water with shaking for 10 minutes.
   (Repeat a total of three times)
3. Remove the solution from step 2, and add 100 mL of Staining Solution. Allow to incubate with shaking for 5 minutes.
   *When disposing of Solution-2 (i.e. Staining Solution), add hydrochloric acid or sodium chloride to the container to precipitate out silver chloride.
4. Remove the solution from step 3, and add 100 mL of deionized water. Incubate with shaking for 30 seconds.
5. Remove the solution from step 4, and add 100 mL of Developer Solution. Incubate with shaking for 30 seconds.
6. Remove the solution from step 5, and add 100 mL of Developer Solution. Allow to incubate with shaking until developed bands appear.
   *Developing time will vary by sample, temperature, and thickness of gel, so adjust accordingly.
   *A 90 mm x 80 mm x 1 mm gel will take approximately 5-10 minutes.
7. Remove the solution from step 6, and add 100 mL of Stop Solution. Incubate with shaking for 10 minutes.
8. Remove the solution from step 7, and wash with 100 mL of deionized water, incubating with shaking for 5 minutes between washes. (Repeat a total of three times.)

Storage

• Store at 4 ºC. This product is photosensitive and should be protected from light. Use within 1 year.