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Product Information

Product number : K0069
Product name : Keratanase II
Quantity : 1.0 U per vial (10 mU/ul)
Product Form : Purified recombinant Keratanase II, Liquid
Storage buffer : 20 mM sodium phosphate buffer, pH7.4
Preservative : None
Stabilizer : None

Description

Keratanase II cleaves the sulfated N-acetylglucosaminidic β1-3 linkage to galactose included in keratan sulfate. The enzyme essentially requires the sulfation at 6-position of the constitutive N-acetylglucosamine. On the other hand, it acts regardless of the sulfation at 6-position of the galactose linked to the sulfated N-acetylglucosamine. The Keratanase II gene is derived from Bacillus circulans and its recombinant protein is expressed in Escherichia coli.

Unit Definition

One unit (U) of Keratanase II is defined as the amount required to produce 1 umol of reducing oligosaccharides from shark cartilage keratan sulfate polysaccharide per minute at 37°C under the condition of pH6.0.

Assay Conditions

Reaction conditions: 20 mM sodium acetate, pH6.0
Reaction mixture includes the enzyme and shark cartilage keratan sulfate in 50 ul. The mixture is incubated at 37°C for 15 min. Quantification of reducing end of released oligosaccharides is measured by the modified Somogyi-Nelson method (see reference).

Storage

Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.
(For long-term use, aliquot and freeze after thawing.)

Related Products

Related oligosaccharides

L0324 L2
L0325 L4
L0286 L4-L4
G0516 L2-L2-β-PEG3-biotin
G0517 L4-L4-β-PEG3-biotin
A3251 Anti-6-sulfo LacNAc Monoclonal Antibody (AG105)
A3252 Anti-6,6'-disulfo LacNAc Monoclonal Antibody (L4L4-8)
A3253 Anti-Sialyl 6,6'-disulfo LacNAc Monoclonal Antibody (G270-16)
A3428 Anti-α2-6-Sialylated 6-sulfo LacNAc Monoclonal Antibody (KN343)

Other oligosaccharide-releasing enzymes

[[Endo-type N-glycanase (ENGase)]]

A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)

[[Endo-type O-glycanase]]

A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)

[[Endo-type glycoceramidase]]

R0240 rEGCase I
R0241 rEGCase I assisted by Activator II
R0242 rEGCase II
R0243 rEGCase II assisted by Activator II