rEGCase II

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纯度/分析方法:

别名：

• rEGCase II
• 重组神经节苷脂内切糖苷酶II
• Recombinant Endoglycoceramidase II
• Recombinant - Rhodococcus sp. EGCase II expressed in Rhodococcus erythropolis

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规格	单价	同一天	2-3个工作日	左侧以上货期	数量	货期信息
1VIAL	S$369.00	5	1	请联系我们		货期预估

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大包装询价

Product Information

• Quantity : 100 mU per vial (2 mU/μL)
• Product Form : Purified recombinant EGCase II, Liquid
• Storage buffer : 20 mM sodium phosphate, 50 mM NaCl buffer, pH 7.0
• Optimum pH : pH 5.5
• Preservative : None
• Stabilizer : None

Description

• Endoglycoceramidase II (EGCase II) is a glycolipid-specific hydrolase that cleaves the glycosidic linkage between oligosaccharide and ceramide of various glycosphingolipids (GSLs) under conditions requiring a detergent or a cofactor Activator II. The EGCase II gene is derived from Rhodococcus sp. and its recombinant protein is expressed in Rhodococcus erythropolis.

Unit Definition

• One unit (U) of EGCase II is defined as the amount required to hydrolyze 1 μmol of ganglioside GM1 per minute at 37°C under the condition of pH 5.5.

Assay Conditions

• Reaction conditions: 50 mM sodium acetate containing 0.4% Triton X-100, pH 5.5
  The reaction mixture includes the enzyme and 100 nmol of GM1 in 50 μL. The mixture is incubated at 37°C for 15 minutes. Quantification of reducing end of released oligosaccharides is measured by the modified Somogyi-Nelson method (see the following reference).
• [Reference]
  Determination of Glucose by a Modification of Somogyi-Nelson Method
  C. Hatanaka, Y. Kobara, Agric. Biol. Chem. 1980, 44, 2943.

Storage

• Store at -20°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 4°C and use it within a few weeks.

Related Products

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• Other oligosaccharide-releasing enzymes
• [Endo-type glycoceramidase]
• R0240 rEGCase I
• R0241 rEGCase I assisted by Activator II
• R0243 rEGCase II assisted by Activator II
• 
  
• [Endo-type N-glycanase (ENGase)]
• A1651 Endo-M (for hydrolysis of non core-fucosylated biantennary N-glycan)
• E1339 Endo-M-W251N (for hydrolysis of core-fucosylated biantennary N-glycan)
• G0365 Glycosynthase (for transglycosylation of non core-fucosylated biantennary N-glycan)
• 
  
• [Endo-type O-glycanase]
• A1844 Endo-α (for hydrolysis of core1 O-glycan epitope known as T/TF antigen)
• G0622 O-Glycosidase for core1 (for hydrolysis of sialylated core1 O-glycan epitope)
• 
  
• [Endo-type glycosaminoglycanase]
• K0069 Keratanase II (for hydrolysis of Keratan sulfate)