Tissue-Clearing Reagent iTOMEI-M (RI 1.40) [for Plants]

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纯度/分析方法:

别名：

• 组织透明剂iTOMEI-M (RI 1.40) [植物用]

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补充产品信息

规格	单价	同一天	2-3个工作日	左侧以上货期	数量	货期信息
5ML	S$42.00	13	1	请联系我们		货期预估
25ML	S$112.50	35	0	请联系我们		货期预估

* 订购TCI产品，请联系我们的经销商或 联系我们。
* TCI会时常优化储存条件，敬请留意。

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大包装询价

"Improved TOMEI (iTOMEI)" is a plant tissue-clearing method that specializes TOMEI for fluorescent protein observation by Prof. Sakamoto et al. iTOMEI enables simply transparency and clearly detecting fluorescent protein.
This product can be used as a mounting agent in iTOMEI. Its refractive index is 1.40, optimized to silicone immersion objectives.

Please see a product brochure (PDF) of how to use.

Description

This product enables transparency by simple osmosis in just a few days (more than 2 days) and keeps the fluorescence of a fluorescent protein such as GFP or tdTomato co-expressed as a reporter gene. The refractive index (RI) of this product is optimized to silicone immersion objectives.
The decoloring solution is also required to complete the iTOMEI method.

Storage

The product should be stored at 4 °C.

Directions for Use

Required reagents

• Paraformaldehyde (PFA)
• PBS
• Stains* (option)

==Process outline (All operations are performed at Room temperature) ===============

• Tissue fixation
  Reagent : 1% PFA in PBS
  Time : 1hr
• PBS Wash ×3
• Decoloring
  Reagent : iTOMEI-D (Product No. T3940)
  Time : 24hr
• PBS Wash ×3
• (Optional process Staining*, PBS Wash ×3)
• Clearing (RI matching)**
  Reagent : iTOMEI-M (Product No. T4003)
  Time : Operation time is determined by the mounting solution.
• Mounting**
  Reagent : iTOMEI-M (Product No. T4003)

*The treatment time of DAPI staining is for 30min with 5 µg/mL and the treatment time of Calcofluor White staining is for 10min with 1 g/L of Calcofluor White M3R and 0.5 g/L of Evans Blue, but their adjustments are needed for the purpose.
** Please use the suitable mounting solution for objective lens.
(iTOMEI-M is optimized to silicone immersion objectives.)

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