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CAS RN: 28718-90-3 | Product Number: A2412
DAPI 2HCl [for Biochemical Research]
Purity: >90.0%(HPLC)
Synonyms:
- 4',6-Diamidino-2-phenylindole Dihydrochloride
- 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride
- 6-Amidino-2-(4-amidinophenyl)indole Dihydrochloride
Product Documents:
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| Product Number | A2412 |
Purity / Analysis Method
|
>90.0%(HPLC) |
| Molecular Formula / Molecular Weight | C__1__6H__1__5N__5·2HCl = 350.25 |
| Physical State (20 deg.C) | Solid |
Storage Temperature
|
Frozen (<0°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
| CAS RN | 28718-90-3 |
| Reaxys Registry Number | 4894417 |
| PubChem Substance ID | 135727109 |
| MDL Number | MFCD00012681 |
Specifications
| Appearance | Light yellow to Yellow to Orange powder to crystal |
| Purity(HPLC) | min. 90.0 area% |
| DNA staining test | to pass test |
Properties (reference)
GHS
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Transport Information:
| H.S.code* | 2933.99-000 |
Application
Fluorescence Staining of Cell Nucleus
DAPI is an organic dye which binds strongly to DNA. DAPI emits a blue fluorescence (Maximum fluorescent wavelength; 461 nm) by the irradiation of UV light (Maximum excitation wavelength; 358 nm).
HeLa cell nucleus stained with DAPI·2HCl [A2412] has been observed by fluorescent microscopy.
HeLa cell nucleus stained with DAPI·2HCl [A2412] has been observed by fluorescent microscopy.

HeLa cells were fixed with 4% paraformaldehyde, and permerabilized with 0.2% Triton X-100.
HeLa cells nuclei were stained with 1 µg/mL DAPI [A2412] solution.
HeLa cells nuclei were stained with 1 µg/mL DAPI [A2412] solution.
References
- Labeling Nuclear DNA Using DAPI
PubMed Literature
Documents
Product Articles
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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![Thumbnail of DAPI 2HCl [for Biochemical Research]](/medias/A2412.jpg?context=bWFzdGVyfHJvb3R8MTM4MDh8aW1hZ2UvanBlZ3xhRGxrTDJnMk5DODRPVE16TVRBME56UTJOVEkyTDBFeU5ERXlMbXB3Wnd8YmUyMWQyMWFmZDVjMTVmYWU3YzAxZGU4MzQ0M2I5N2I0MDEwOThkZWM0Njg2MTJjNGM0NWM1Mzc2ZTM0ZjNmZg)
![Thumbnail of DAPI 2HCl [for Biochemical Research]](/medias/Tci-98-A2412-DAPI-2HCl-was-used-to-stain-the-nuclei.jpg?context=bWFzdGVyfHBkcC1hcHBsaWNhdGlvbi1pbWFnZXN8MzA5MnxpbWFnZS9qcGVnfGFHUTRMMmhpWkM4NU5URTBNVE14TXprd05EazBMMVJqYVMwNU9GOUJNalF4TWkxRVFWQkpMVEpJUTJ3dGQyRnpMWFZ6WldRdGRHOHRjM1JoYVc0dGRHaGxMVzUxWTJ4bGFTNXFjR2N8MDc2NDFiMWYzZjk5ODljMTViZWMyNDE5YWQ2ZTg2OWNjNzI1ZTE5NDVmMzBlOWMyYzAxMmQ0YTBlODE0M2Q1Zg)