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CAS RN: | Product Number: B5702
Bradford Assay Solution (Ready-to-use) [for Protein determination]
Purity:
Synonyms:
- Coomassie Brilliant Blue G-250 (Ready-to-use)
- Acid Blue 90 (Ready-to-use)
- Brilliant Blue G (Ready-to-use)
- CBB G-250 (Ready-to-use)
Product Documents:
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Supplemental Product Information:
Product Information
- Volume : 500 mL
Description
- This product is supplied as a ready-to-use solution for protein assay based on the method of Bradford. This product contains Coomassie Brilliant Blue G-250 (CBB G-250). When the dye containing CBB G-250 binds proteins, the absorption maximum of the dye shifts from 465 to 595 nm linearly with the quantity of the protein. This product requires protein standard solution (such as BSA).
Direction for Use
- See Application (PDF).
Related Products
- T3796 Standard Solution of Albumin from Bovine Serum
| Product Number | B5702 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Green to Dark green to Dark blue clear liquid |
| Protein determination test | to pass test |
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Danger |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. H360 : May damage fertility or the unborn child. H372 : Causes damage to organs through prolonged or repeated exposure. H373 : May cause damage to organs through prolonged or repeated exposure. H340 : May cause genetic defects. H350 : May cause cancer. H225 : Highly flammable liquid and vapor. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P270 : Do not eat, drink or smoke when using this product. P202 : Do not handle until all safety precautions have been read and understood. P240 : Ground and bond container and receiving equipment. P260 : Do not breathe mist or vapours. P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P233 : Keep container tightly closed. P201 : Obtain special instructions before use. P243 : Take action to prevent static discharges. P241 : Use explosion-proof electrical/ ventilating/ lighting/ equipment. P242 : Use non-sparking tools. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection. P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish. P308 + P313 : IF exposed or concerned: Get medical advice/ attention. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P362 + P364 : Take off contaminated clothing and wash it before reuse. P303 + P361 + P353 : IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. P403 + P235 : Store in a well-ventilated place. Keep cool. P405 : Store locked up. |
Related Laws:
Transport Information:
| H.S.code* | 3822.19-000 |
Application
Bradford Assay Solution for Quantification of Protein Concentration
Figure. Staining a dilution series of BSA by Bradford assay solution
References
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
- An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination
Application
Bradford Assay Solution (Ready-to-use) [for Protein determination]
Reference
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Documents
Product Articles
[Product Highlights] Bradford Assay Solution for Quantification of Protein ConcentrationProduct Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
C of A & Other Certificates
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Analytical Charts
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