text.skipToContent text.skipToNavigation

Maximum quantity allowed is 999

Please select the quantity

CAS RN: | Product Number: R0195

Resazurin (Ready-to-use solution) [for Cell proliferation assay]

≥20  Contact Us

* Please contact our distributors or TCI to order our products. The above prices do not include freight cost, customs, and other charges to the destination.
* The storage conditions are subject to change without notice.


Product Information
Volume: 25 mL
Product Form: Ready-to-use solution

Resazurin can be used quantitatively determine cell proliferation, viability, and cytotoxicity. Resazurin, when added to viable cells, is reduced by the cellular enzymatic or chemical reactions converting blue/non-fluorescent resazurin to highly fluorescent resorufin.
The assay is simple to perform since the indicator is water-soluble and has low toxicity, thus eliminating the washing/fixing and extraction steps required in other commonly used cell proliferation assays.

Direction for Use
Resazurin may be added at any time point during the culture period. For measurement of cell proliferation, it is best to add resazurin during the log phase of growth.
1. In a sterile work area, add resazurin ready-to-use solution at a volume equal to 10% of the cell culture media volume.
2. Return cells to the incubator and continue the incubation for 2-24 hours*.
*Incubation times may vary depending on the metabolic rates of the cell lines being tested.
3. Measure the fluorescent intensity using 540-570 nm excitation and 590 nm emission wavelengths. Absorbance can be measured using a spectrophotometer set at 570 nm.

The product is shipped at ambient temperature. Upon receipt the product should be stored at 4 °C, protected from light, and used within 6 months. Avoid repeated freezing and thawing.

Related Products
A2412 2-(4-Amidinophenyl)indole-6-carboxamidine Dihydrochloride [for Biochemical Research]
H1343 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride Hydrate [for Biochemical Research]
T0556 Direct Blue 14 (Trypan Blue)

Product Number R0195
Physical State (20 deg.C) Liquid
Storage Temperature -20°C
Condition to Avoid Heat Sensitive
Appearance Blue to Purple clear liquid
Correlation between fluorescence and cell number to pass test
Properties (reference)
Related Laws:
Transport Information:
H.S.code* 3822.19-000
*This code is applied to the products when TCI exports from Japan and not for import in your country.
Resazurin (Ready-to-use solution) [for Cell proliferation assay]


Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
Please select Language.

The requested SDS is not available.

Please Contact Us for more information.

C of A & Other Certificates
Please enter Lot Number Incorrect Lot Number is entered
Analytical Charts
Please enter Lot Number Incorrect Lot Number is entered

The requested analytical chart is not available. Sorry for the inconvenience.

Other Documents