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CAS RN: | Product Number: R0232

rMOA-Biotin [for Galα(1-3)Gal]


Purity:
Synonyms:
  • rMOA-Biotin Conjugate
  • Recombinant Marasmius oreades Agglutinin (= rMOA)-Biotin Conjugate
  • Recombinant Marasmius oreades lectin (= rMOA)-Biotin Conjugate
Documents:
1ML
$328.00
7   1  

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* The storage conditions are subject to change without notice.


Note:

Product Information
Product code: R0232
Product name: rMOA-Biotin [for Galα(1-3)Gal]
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Biotin-immobilized recombinant MOA, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: >32 kDa
Preservative : None
Stabilizer: None

Description
The rMOA is a carbohydrate-binding protein (Lectin) derived from Marasmius oreades. The rMOA gene product is expressed in Escherichia coli. This lectin preferably binds to a [Galα(1-3)Gal] glycan epitope.
Biotin is covalently linked to the protein surface. Resulting band shift caused by biotinylation is checked by SDS-PAGE. (Unbiotinylated band is mostly not observed.) The rMOA-Biotin shows significant interaction with Cetuximab possessing [Galα(1-3)Gal] glycan epitopes by lectin-ELISA analysis. According to the lectin-blotting analysis, the rMOA-Biotin specifically binds to α(1-3)Gal-terminated Cetuximab but not to non-α(1-3)galactosylated glycoproteins such as human transferrin (hTF), asialo hTF, agalacto hTF and bovine RNase B.

Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it’s recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).

Related Products
R0227 Recombinant Marasmius oreades agglutinin (= rMOA) expressed in Escherichia coli
R0237 rMOA-LecBeads [for Galα(1-3)Gal]
R0230 rPSL1a-Biotin [for Sia α(2-6)Gal]
R0231 rLSL-N-Biotin [for Galβ(1-4)GlcNAc, poly LacNAc]
R0233 rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0234 rGRFT-Biotin [for Manα(1-2)Man]
A2659 AOL-Biotin Conjugate

Product Number R0232
Physical State (20 deg.C) Liquid
Storage Temperature -20°C
Condition to Avoid Heat Sensitive
Specifications
Appearance Colorless to Almost colorless clear liquid
Concentration(Lowry method) 0.9 to 1.5 mg/mL
Molecular weight 25 to 40 kDa
Suitability for use in ELISA tests min. 1.0 (1.0 micro g/mL, OD450)
Properties (reference)
GHS
Related Laws:
Transport Information:
H.S.code* 3504.00-000
*This code is applied to the products when TCI exports from Japan and not for import in your country.
Application
Recombinant lectin

References


TCIMAIL
Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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