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CAS RN: | Product Number: D6540

DiR Solution [for Cell Membrane Staining]

Chemical Structure of DiR Solution [for Cell Membrane Staining]
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× DiR Solution [for Cell Membrane Staining]

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Supplemental Product Information

Explanatory notes for this page

1ML
€250.00
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*Stock available in Belgium will be delivered in 1 to 3 days
*Stock available in Japan will be delivered in 1 to 2 weeks (excludes regulated items and dry ice shipments).

Supplemental Product Information:

Product Information

  • Concentration : 1 mM
  • Excitation / Emission : 757 nm / 781 nm

Description

  • DiR is a lipophilic carbocyanine dye used to label cell membranes. It emits strong fluorescence when its long hydrocarbon chains are incorporated into a lipid bilayer. This product is a ready-to-use solution of DiR which can be used as a tracer in cell fusion, cell adhesion and chemotaxis.

Storage

  • Store at room temperature (below 15 °C is recommended) and protect from light.

Directions for Use: Staining live cells

    1. Dilute DiR solution to 5 μM with culture medium.
    2. Remove medium from cells and replace with 5 μM DiR-containing medium.
    3. Incubate cells at 37 °C for 10 minutes.
    4. Remove medium and wash cells 3 times with PBS.
    5. Observe stained cell membranes via a fluorescence microscope.

Directions for Use: Staining fixed cells

    1. Remove culture medium and wash cells twice with PBS.
    2. Add 4% paraformaldehyde to cells and incubate at room temperature for 10 minutes.
    3. Dilute DiR solution to 5 μM with PBS.
    4. Remove 4% paraformaldehyde and add 5 μM DiR-containing PBS to cells.
    5. Incubate cells at 37 °C for 10 minutes.
    6. Remove medium and wash cells 3 times with PBS.
    7. Observe stained cell membranes via a fluorescence microscope.

Related Products

  • D6202 DiI Solution [for Cell Membrane Staining]
  • D6263 DiD Solution [for Cell Membrane Staining]
  • D6539 DiO Solution [for Cell Membrane Staining]
  • P3316 PKH 26 Solution [for Cell Membrane Staining]

Product Number D6540
Physical State (20 deg.C) Liquid
Storage Temperature Room Temperature (Recommended in a cool and dark place, <15°C)
Store Under Inert Gas Store under inert gas
Condition to Avoid Light Sensitive,Air Sensitive
Specifications
Appearance Dark red to Dark purple to Dark blue powder to crystal
Functionality test to pass test
Properties (reference)
GHS
Pictogram Pictogram Pictogram Pictogram
Signal Word Danger
Hazard Statements H319 : Causes serious eye irritation.
H360 : May damage fertility or the unborn child.
H372 : Causes damage to organs through prolonged or repeated exposure.
H350 : May cause cancer.
H225 : Highly flammable liquid and vapour.
Precautionary Statements P260 : Do not breathe mist or vapours.
P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P201 : Obtain special instructions before use.
P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
P308 + P313 : IF exposed or concerned: Get medical advice/ attention.
Related Laws:
Transport Information:
UN Number UN1170
Class 3
Packing Group II
HS Number 3822190090
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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