Organosilica Rhodamine B (100nm Diam.)

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Supplemental Product Information

Size	Unit Price	Same Day	2-3 Business Days	Other Lead Time	Shipping Information
2MG	NT$7,644	31	0	Contact Us	Shipping Simulation

* The above prices include freight cost, customs, and other charges to the destination except for products that need to be shipped by sea or dry ice. For details, please contact our distributor in Taiwan to order our product.
* The storage conditions are subject to change without notice.

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Product Information

• Volume/Quantity : 2 mg
• Product Form : Suspension in water
• Functional Group : Thiol group (-SH)
• Excitation / Emission : 556 nm / 579 nm

Application

• For use as a fluorophore in the fluorescent labeling of proteins and other molecules. Incorporation of Rhodamine B fluorescent dye into conjugatable silica nanoparticles allows for superior fluorescence intensity and light resistance when compared to unmodified Rhodamine B. Thiol groups (-SH) at the surface of the nanoparticles provide a convenient mechanism for conjugation. This product is made without toxic metals.

Storage

• Store at 4 °C. Do not freeze.

Direction for Use

• 1. Prepare 10 mM linker solution (For examples of linkers, see Related Products).
     e.g. Dissolve 2.8 mg of GMBS (Product No. S0399) in 1 mL of DMSO (Product No. D0798).
  2. Mix 10 mM linker solution with appropriate volume of antibody. (Suggested linker:antibody molar ratio = 100:1)
     e.g. Mix 100 µL of 1.5 mg/mL (= 10 µM) antibody solution with 10 µL of 10 mM linker solution.
  3. Incubate linker-antibody solution for 1 hour at room temperature.
  4. Remove unreacted and/or hydrolyzed reagent using a desalting column or dialysis method (not included).
  5. Mix 1 part by weight purified antibody-linker solution with 1.5 parts by weight Organosilica Rhodamine B (Product No. O0573).
     e.g. For the antibody-linker solution described in step 2 (antibody weight approx. 0.15 mg), use 45 µL of 5.0 mg/mL Organosilica Rhodamine B.
  6. Incubate the antibody-nanoparticle solution for 1 hour at room temperature.
  7. Collect conjugated nanoparticles via centrifugation (20,000 x g, 4 °C, 10 minutes) and wash with 500 µL deionized water. Repeat wash step two additional times. Resuspend final washed pellet in deionized water.

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