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CAS RN: | Product Number: R0228
Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
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| 1ML |
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[Handling Notes]
Avoid multiple freeze-thaw cycles. Add some preservative, e.g. sodium azide upon opening for the first time, and store at 2-10 °C.
Product Information
Product code: R0228
Product name: Recombinant Sclerotium rolfsii lectin (= rSRL) expressed in Escherichia coli
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Purified recombinant SRL, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: 16 kDa
Preservative : None
Stabilizer: None
Description
The rSRL is a carbohydrate-binding protein (Lectin) derived from Sclerotium rolfsii. The rSRL gene product is expressed in Escherichia coli. This lectin preferably binds to two types [GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc] of glycan epitopes by its two distinct carbohydrate-binding sites. Consequently, rSRL exhibits a hemagglutination activity to a rabbit reticulocyte through the glycan binding to subsites and subunits of the lectin.
Storage
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it's recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
Related Products
R0233 rSRL-Biotin [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0238 rSRL-LecBeads [for GlcNAcβ(1-2)Man, Galβ(1-3)GalNAc]
R0225 Recombinant Polyporus squamosus lectin (=rPSL1a) expressed in Escherichia coli
R0226 Recombinant Laetiporus sulphureus lectin N-Terminal Domain (= rLSL-N) expressed in Escherichia coli
R0227 Recombinant Marasmius oreades agglutinin (= rMOA) expressed in Escherichia coli
R0229 Recombinant Griffithsia sp. lectin (= rGRFT) expressed in Escherichia coli
L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae (5mg/mL, PBS pH6.5)
| Product Number | R0228 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Frozen (-20°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Light yellow to Light orange clear liquid |
| Concentration(Lowry method) | 0.9 to 1.5 mg/mL |
| Molecular weight | 10000 to 25000(SDS-PAGE) |
| Hemagglutination activity | max. 12.5 micro g/mL(pH7.2) |
Properties (reference)
GHS
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Transport Information:
| H.S.code* | 3504.00-000 |
Application
Recombinant lectin
References
- A newly isolated lectin from the plant pathogenic fungus Sclerotium rolfsii: purification, characterization and role in mycoparasitism
- Carbohydrate specificity of a lectin isolated from the fungus Sclerotium rolfsii
- Structural basis for the carbohydrate recognition of the Sclerotium rolfsii lectin
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