Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]

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包裝	價格	同一天	2-3个工作日	左側以上貨期	貨期資訊
1KIT	NT$6,975	≥40	0	聯繫我們	貨期預估

* 以上價格已含運費關稅等但一些需要海運以及乾冰運輸的產品除外,詳情請與 當地經銷商 洽詢。
* TCI會時常優化儲存條件,儲存溫度請以在線目錄為準，敬請留意。

大包裝詢價

Product Information

• Components : Chemiluminescence HRP Substrate Solution A 100 mL×1, Chemiluminescence HRP Substrate Solution B 100 mL×1
• Volume : 1 kit (for 2000 cm² of membrane)

Description

• This product is a reagent used for the chemiluminescent detection of membrane-transferred HRP-labeled proteins in Western blotting. Immediately before use, solutions A and B are mixed 1:1 and applied to the membrane. Proteins can be detected down to nanogram level.

Storage

• The product should be stored at 4 °C and protected from light.

Direction for Use

• 1. Bring Solution A and Solution B to room temperature.
  2. Prepare a western blot membrane labeled with an HRP-conjugated probe.
  3. Wash the membrane three times for 10 minutes each time with 1× TBS-T buffer.
  4. Mix Solution A and Solution B in a 1:1 ratio immediately before use. The recommended volume is 0.1 mL/cm² membrane.
  5. Place the membrane protein side up on a sheet of the plastic wrap or polyethylene sheet.
  6. Gently pour the mixture evenly over the surface of the membrane.
  7. Incubate for 60 seconds at room temperature.
  8. Remove excess reagent by blotting with a tissue or tilting the membrane upright to allow excess to run off.
  9. Remove excess reagent by blotting with a tissue or tilting the membrane upright to allow excess to run off.
  10. Image the membrane using an imaging system or X-ray film.

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