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CAS RN: | 產品號碼: M3353

MTT Solution [for Cell proliferation assay] (1mL×5)


纯度/分析方法:
別名
  • 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium Bromide Solution (1mL×5)
文件:
1SET
NT$3,200
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補充產品訊息:

Product Information

Volume/Quantity : 1mL x 5
Product Form : 5.0 mg / mL in PBS

Description

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide (MTT) is a type of tetrazolium salt that changes to formazan when taken up into living cells. As formazan is a non-water soluble crystal, it can be dissolved in DMSO and the absorbance at 570 nm can be used to count the number of cells. This product consists of 5.0 mg / mL MTT dissolved in PBS. When 1/10th of the medium is added to cultured cells, the live cells are properly stained.


Storage

This product should be stored at -20 °C and should be protected from light. Avoid repeated freezing and thawing.


Directions for Use

MTT solution can be added at any point in the cell culture. For best results, it should be added to cells in the logarithmic growth phase. Pre-determine the number of cells and seed 10,000 to 100,000 cells per well in a 96-well plate.

1. Seed 100 μL of cells in a 96-well plate.
2. Incubate overnight in an incubator.
3. Add 10 μL of MTT solution to each well.
4. Incubate for 2-4 hours for color reaction.
5. The medium is removed, taking care not to inhale the formazan.
6. Add 100 μL of DMSO (Product No. D0798) and dissolve the formazan.
7. Measure the absorbance at 570 nm.


Related Products

R0195 Resazurin (Ready-to-use solution) [for Cell proliferation assay]
D0798 Dimethyl Sulfoxide
M3297 MTT [for Biochemical Research]

產品號碼 M3353
外觀與形狀(20°C) Liquid
儲存條件 Frozen (-20°C)
應避免的情況 Heat Sensitive
產品規格
Appearance Light yellow to Yellow to Orange clear liquid
Cell staining test to pass test (min. 0.5, Abs at 540nm)
性質
GHS
相關法規
運輸資料
HS編碼* 3822.19-000
*此H.S.編碼用於日本出口報關, 不適用於您所在國家或地區的進口申報
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