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CAS RN: | 產品號碼: M3637
Malondialdehyde Measurement Kit
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補充產品訊息:
Product Information
- Components : Acetic Acid 1 vial, Butylhydroxytoluene (BHT) solution 1 vial, Malonaldehyde Bis(dimethyl Acetal) (1 mM MDA solution) 1 vial, 2-Thiobarbituric Acid (TBH) 2 vials
- Size : 100 tests
Description
- This product allows determination of malondialdehyde content of your sample through thiobarbituric acid reactive substances (TBARS) method.
Direction for Use (for Measurement between 1 – 50 µM of Malondialdehyde)
-
- Prepare a sample.
- Mix 50 µL of 1 mM MDA solution and 950 µL of deionized water to obtain a 50 µM solution of malondialdehyde.
- Prepare a 2x dilution series of the malondialdehyde solution from step 2 with 7 points total (0.78125 mM – 50 mM) of at least 250 µL each, to allow for duplicates. Deionized water should be prepared as a blank.
- Add 600 µL of DMSO to one of the vials of 2-Thiobarbituric Acid to dissolve. Accelerate the dissolution by voltexing or ultrasonic bath if the powder does not dissolve.
- Add 5.5 mL of Acetic Acid to the tube prepared in step 4 to prepare a 50 mM TBH solution.
- Add 25 µL of BHT solution and 100 µL of 50 mM TBH solution to 100 µL of each sample / point in the dilution series from step 3.
- Incubate at 95 °C for 15 minutes.
- Measure the absorbance at 532 nm.
- Create a standard curve to calculate the MDA concentration in the sample.
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