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CAS RN: | Product Number: A3176

AzBTS (Ready-to-use solution) [for ELISA]

  • 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt) (Ready-to-use solution)
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Product Information

Volume : 100 mL


This product is supplied as a ready-to-use solution containing 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt) (= AzBTS) and hydrogen peroxide (H2O2) for ELISA. When this product is reacted with horseradish peroxidase (HRP), a green soluble reaction product appears. Its absorbance can be measured at 405 nm.

Direction for Use

The following steps are for a 96-well plate format.

  1. Bring AzBTS solution (Product No. A3176) to room temperature.
  2. Gently mix the AzBTS solution (Product No. A3176).
  3. Add 100μL of AzBTS solution (Product No. A3176) to each well.
  4. Incubate the plate at room temperature for 30 minutes.
  5. Within 1 hour from start the reaction, measure the absorbance of each well at 405 nm.


The product should be stored at 4 °C, and used within 6 months. Don’t freeze because of arising precipitate.

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Product Number A3176
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Heat Sensitive
Peroxidase detection test Extent of coloring same as reference
Properties (reference)
Pictogram Pictogram Pictogram
Signal Word Danger
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
H370 : Causes damage to organs.
H372 : Causes damage to organs through prolonged or repeated exposure.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P260 : Do not breathe dust/ fume/ gas/ mist/ vapors/ spray.
P270 : Do not eat, drink or smoke when using this product.
P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P307 + P311 : IF exposed: Call a POISON CENTER or doctor/ physician.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
P405 : Store locked up.
Related Laws:
Transport Information:
HS Number 3822.00.1090
AzBTS (Ready-to-use solution) [for ELISA]


  • Cold Spring Harbor Laboratory Press, New York
    E. Harlow, D. Lane. Antibodies, A Laboratory Manual, 1988, p404, p506, p594.

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