text.skipToContent text.skipToNavigation

Maximum quantity allowed is 999

Please select the quantity

CAS RN: 65-61-2 | Product Number: A3396

Acridine Orange Solution [for Cell Staining]


Purity:
Synonyms:
Documents:
5ML
$42.00
Contact Us Contact Us ≥40 

* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
* To send your quote request for bulk quantities, please click on the "Request Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.


Note:

Product Information
Volume : 5 mL
Product Form : 0.05 mg/mL solution

Description
Acridine orange is a cell membrane-permeant nucleic acid cell stain, which is incorporated into double-stranded DNA at a ratio of one to three base pairs. It fluoresces at 520nm (Ex. 500nm) when bound to double stranded DNA, and 650nm (Ex. 460nm) when bound to RNA or single-stranded DNA. As acridine orange is mutagenic, this product is supplied as a pre-made solution to prevent exposure during weighing.

Direction for Use
Adjust volume and staining time as necessary. Because some cells need to be fixed prior to staining, preliminary testing should be performed.
1. Remove medium from cells and wash twice with cold PBS(-).
2. Remove PBS(-) and add an appropriate volume of 1:50 Acridine Orange (Product No. A3396) in PBS(-). Allow to stain for 15 minutes.
3. Remove the staining solution and wash twice with PBS(-).
4. Observe cells with a fluorescence microscope while submerged in PBS(-).

Storage
Upon receipt the product should be stored at 4 °C, protected from light, and used within 6 months.

Related Products
R0195 Resazurin (Ready-to-use solution) [for Cell proliferation assay]
R0246 RIPA Buffer (Ready-to-use) [for Protein extraction]
O0483 Oil Red O [for Biochemical Research]

Product Number A3396
Molecular Formula / Molecular Weight C__1__7H__1__9N__3·HCl = 301.82 
Physical State (20 deg.C) Liquid
Storage Temperature 0-10°C
Condition to Avoid Light Sensitive,Heat Sensitive
CAS RN 65-61-2
PubChem Substance ID 468590276
MDL Number

MFCD00012660

Specifications
Appearance Light yellow to Brown clear liquid
DNA staining test to pass test
Properties (reference)
GHS
Related Laws:
RTECS# AR7601000
Transport Information:
HS Number 3204.19.5000
Application
Cell Staining

References


TCIMAIL
Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
Please select Language.

The requested SDS is not available.

Please Contact Us for more information.

Specifications
C of A & Other Certificates
Please enter Lot Number Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Analytical Charts
Please enter Lot Number Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.

The requested analytical chart is not available. Sorry for the inconvenience.

Other Documents

Session Status
Your session will timeout in 10 minutes. You will be redirected to the HOME page after session timeout. Please click the button to continue session from the same page. minute. You will be redirected to the HOME page after session timeout. Please click the button to continue session from the same page.

Your session has timed out. You will be redirected to the HOME page.