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CAS RN: 139332-66-4 | Product Number: A5554

NBD-PZ (=4-Nitro-7-piperazino-2,1,3-benzoxadiazole) [for HPLC Labeling]


Purity: >98.0%(T)(HPLC)
Synonyms:
  • 4-Nitro-7-piperazinobenzofurazan
  • 4-Nitro-7-piperazino-2,1,3-benzoxadiazole
Documents:
100MG
$119.00
7   3   ≥20 

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Product Number A5554
Purity / Analysis Method >98.0%(T)(HPLC)
Molecular Formula / Molecular Weight C__1__0H__1__1N__5O__3 = 249.23  
Physical State (20 deg.C) Solid
CAS RN 139332-66-4
Reaxys Registry Number 7936339
PubChem Substance ID 87563172
MDL Number

MFCD00191507

Specifications
Appearance Dark red to Brown powder to crystal
Purity(HPLC) min. 98.0 area%
Purity(Nonaqueous Titration) min. 98.0 %
Effect test of HPLC derivatization Effective as labeling agent for Linoleic Acid , Oleic Acid
Properties (reference)
Melting Point 210 °C(dec.)
GHS
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P302 + P352 : IF ON SKIN: Wash with plenty of soap and water.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
P362 : Take off contaminated clothing and wash before reuse.
Related Laws:
Transport Information:
HS Number 2934.99.9001
Application
HPLC Labeling Reagent for Carboxylic Acids

The compound 1 is an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a piperazino group, easily reacts with a carboxyl group at room temperature to form the corresponding amide in the presence of a condensation reagent. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 470 nm and 541 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference by contaminants. A highly sensitive detection can be done by using laser induced fluorescence detector.

Application examples:
[Fatty acids] 1)
0.2 mL of 140 mM DEPC or 70 mM 2,2’-dipyridyl disulfide-Ph3P / DMF solution containing a fatty acid (10 µM) is added to 0.2 mL of the labeling reagent 1 / DMF or acetonitrile solution (10 mM). React at room temperature for 6 h, then use it as an HPLC sample.

References


PubMed Literature


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