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CAS RN: | Product Number: I1275
Clearing Reagent iCBiofilm-D [for Biofilm]
Purity:
Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
---|---|---|---|---|---|
8ML |
$120.00
|
Contact Us | Contact Us | 20 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
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*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
- Size : 8 mL
- Sterility : Filter sterilized
Description
- Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms. Clearing Reagent iCBiofilm-D has been developed to allow for live-cell-imaging of biofilm at thicknesses of more than several tens of micrometers. Simple addition of iCBiofilm-D solution to a biofilm renders it transparent within several seconds, allowing for easy observation using light microscopy techniques. This product has been filter-sterilized.
*This product was developed by Associate Prof. Sugimoto.
Storage
- Store at room temperature.
Note
-
- For Research Use Only. Not for Diagnostic or Therapeutic Use.
- Vigorous manipulation of this product may cause biofilm to peel off.
- 24-hour live cell imaging of biofilm can be observed.
- If clear images cannot be obtained or biofilm formation is prevented in iCBiofilm-D, try diluting iCBiofilm-D twice by culture medium.
Directions for Use (for live-cell-imaging of biofilm)
-
- Prepare a live-cell-imaging medium. Dissolve the culture medium powder in iCBiofilm-D to create a biofilm culture medium by autoclaving and so on.
- Prepare a biofilm in a glass bottomed culture dish (35mm diam.) in ordinary medium (not live-cell-imaging medium). Add staining reagents as needed. Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
- After removing the culture medium, wash the dish with the ordinary medium to remove planktonic cells.
- After wash, add the live-cell-imaging medium. Add staining reagents as needed.
- Observe the biofilm and obtain the images of live-cell-imaging.
Related Products
- T4031 Clearing Reagent iCBiofilm-H1 [for Biofilm]
- T4032 Clearing Reagent iCBiofilm-H2 [for Biofilm]
Product Number | I1275 |
Physical State (20 deg.C) | Liquid |
Storage Temperature | Room Temperature (Recommended in a cool and dark place, <15°C) |
Appearance | Colorless to Light yellow clear liquid |
Refractive index | 1.3900 to 1.4200 |
Functionality test | to pass test |
HS Number | 3822.19.0080 |
Articles/Brochures
Safety Data Sheet (SDS)
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Specifications
C of A & Other Certificates
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