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Core-fucosylated N-Glycan and Labeled Core-fucosylated N-Glycan based on Chemical Synthesis

Core-fucosylated N-Glycan

Our chemical synthesis of oligosaccharide using various sugar building blocks provides human type N-glycans (G0, uniform isomers 3-G1 and 6-G1, and G2) with a core fucose bound to a position 6 of the reducing terminal β-N-acetylglucosamine.

Core-fucosylated N-Glycan

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Labeled Core-fucosylated N-Glycan

We produce high grade 2-AB labeled core-fucosylated N-glycans. Please take advantage of our fluorescent-labeled products as a standard glycan for MS, CE and HPLC analyses of biopharmaceuticals including antibody drugs.

Labeled Core-fucosylated N-Glycan

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Separation of Labeled Core-fucosylated N-Glycan

(a) The labeled N-glycan mixture was separated based on the distinctive features of glycan structure. (However, G1 and G1F isomers were not individually separated.)
(b) The G1 isomers (3-G1 and 6-G1) and G1F isomers (3-G1F and 6-G1F) are modestly detached in this condition.

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MS / HPLC Data of Labeled Core-fucosylated N-Glycan

MS / HPLC Data of Labeled Core-fucosylated N-Glycan

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NMR Data of Labeled Core-fucosylated N-Glycan

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This research is partially supported by the developing key technologies for discovering and manufacturing pharmaceuticals used for next-generation treatments and diagnoses both from the Ministry of Economy, Trade and Industry, Japan (METI) and from Japan Agency for Medical Research and Development (AMED). This research was supported by AMED under Grant Number JP17ae0101003.

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