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DAB Staining Kit to Detect Peroxidase
3,3'-Diaminobenzidine is an enzymatic substrate that reacts with horseradish peroxidase (HRP) in the presence of hydrogen peroxide to form a brown precipitate that is insoluble in water. DAB is used in dot blotting western blotting and immunohistochemical staining to detect HRP-labeled antibodies.1,2) DAB staining kit includes DAB solution, hydrogen peroxide solution, and reaction buffer for easy use. Each reagent is contained in a dropper bottle, which both allows you to add a fixed volume of reagent to the reaction and helps prevent chemical exposure.
Figure. Dot blot image of substrate solutions prepared by DAB staining kit reacting with HRP (from left to right: 10, 5, 2.5, 1.25, 0.625, 0.312, 0.156, 0.078 (mU/spot)).
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References
- 1) Assessment of a method for immunochemical detection of antigen on nitrocellulose membranes
- 2) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures
