RNA Structure Analysis Reagent for Sulfonylation of 2'-OH Group of RNA, Stable in Aqueous Solvents

P3S is an RNA structural mapping reagent that sulfonylates the 2'-OH groups of ribonucleotides in unpaired regions of RNA.¹⁾ P3S has advantages such as good solubility in aqueous solvents and a long half-life in water. In addition, P3S shows a different modification pattern from SHAPE (Selective 2'-Hydroxyl Acylation and Primer Extension) reagents,²⁾ which acylate 2'-OH groups like P3S and have been employed for the analysis of RNA structural characteristics. Thus, it is expected that a new structural analysis using P3S will lead to the development of applications for RNA.

Related Products

P3153

P3S

Related Product Category Pages

• RNA Structure Analysis

References

• 1) Sulfonylation of RNA 2'-OH groups
  • S. Chatterjee, R. Shioi, E. T. Kool, ACS Cent. Sci. 2023, 9, 531.
• 2) RNA Structure Analysis at Single Nucleotide Resolution by Selective 2'-Hydroxyl Acylation and Primer Extension (SHAPE)
  • E. J. Merino, K. A. Wilkinson, J. L. Coughlan, K. M. Weeks, J. Am. Chem. Soc. 2005, 127, 4223.

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