CUBIC-L, X1, and X2 for Observation of Biological Samples Using Expansion Microscopy

Expansion microscopy is an experimental technique reported by Boyden et al. in 2015. In this method, biological samples are fixed, forming a hydrogel, and then physically expanded by taking advantages of changes in solvent properties, which allows for easier observation.¹⁾ With CUBIC tissue-clearing reagents, biological samples can be cleared with expansion by simple sequential immersion in CUBIC-L, CUBIC-X1, and CUBIC-X2, without gel fixation. Using this approach, a mouse brain expands to approximately 2.2 times its original length²⁾ and about 10 times its original volume.³⁾ Tissue clearing by expansion microscopy using CUBIC reagents enables observation of biological samples while preserving intracellular structures without destruction.³⁾

Related Products

T3740

Tissue-Clearing Reagent CUBIC-L

T3866

Tissue-Clearing Reagent CUBIC-X1

T3867

Tissue-Clearing Reagent CUBIC-X2

F0622

Formaldehyde Solution (37%)

Related Product Category Page

• Animal Tissue-Clearing Reagents

References

• 1) Optical imaging. Expansion microscopy
  • F. Chen, P. W. Tillberg, E. S. Boyden, Science 2015, 347, 543.
• 2) Advanced CUBIC tissue clearing for whole-organ cell profiling
  • K. Matsumoto, T. T. Mitani, S. A. Horiguchi, J. Kaneshiro, T. C. Murakami, T. Mano, H. Fujishima, A. Konno, T. M. Watanabe, H. Hirai, H. R. Ueda, Nat. Protoc. 2019, 14, 3506.
• 3) A three-dimensional single-cell-resolution whole-brain atlas using CUBIC-X expansion microscopy and tissue clearing
  • T. C. Murakami, T. Mano, S. Saikawa, S. A. Horiguchi, D. Shigeta, K. Baba, H. Sekiya, Y. Shimizu, K. F. Tanaka, H. Kiyonari, M. Iino, H. Mochizuki, K. Tainaka, H. R. Ueda, Nat. Neurosci. 2018, 21, 625.

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