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Mitochondrial Membrane Potential Detection Reagent
JC-1 is a cationic fluorescent dye widely used for the evaluation of mitochondrial membrane potential. In healthy mitochondria with an intact membrane potential, JC-1 forms aggregates and emits red fluorescence. However, in mitochondria with a decreased membrane potential, JC-1 remains in its monomeric form and emits green fluorescence.1,2) As such, changes in the ratio of red and green fluorescence can be used to assess the functional state of mitochondria (Figure). This product is supplied as a powder that can be prepared in accordance with the requirements of your specific research application.
Figure. Mitochondrial membrane potential in cells treated with CCCP, observed by fluorescence microscopy. All of these photos were taken in-house.
CCCP, a mitochondrial uncoupler, induced a concentration-dependent reduction in mitochondrial membrane potential.
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References
- 1) J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential
- 2) JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry
