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Printed Magazine "TCIMAIL" > TCIMAIL Back Numbers 2025 >

Silver Staining Kits for Staining Proteins and DNA after Electrophoresis

No.199(August 2025)
I1309
Silver Stain Kit [for Electrophoresis] (1)

Silver staining is a typical method used to detect proteins and DNA acids in polyacrylamide gels after electrophoresis.1) Silver ions bind to proteins and DNA in the gel and are then reduced, resulting in a silver-stained image.2) 1 is more sensitive than Coomassie Brilliant Blue (CBB) staining, and can detect nanogram amounts of proteins. Also, it can stain proteins and DNA in gels in about one hour (Figures A-C). In addition, it is safe to use since it is ammonium free and does not form explosive silver amides.

Figure A. Protein molecular weight markers were diluted, run on an acrylamide gel, and stained with 1
Figure A. Protein molecular weight markers were diluted, run on an acrylamide gel, and stained with 1
(from left, 500 ng/lane, 250 ng/lane, 150 ng/lane, 50 ng/lane, 15 ng/lane, 5 ng/lane and 1 ng/lane)
 
Figure B. BSA was diluted, run on an acrylamide gel, and stained with 1
Figure B. BSA was diluted, run on an acrylamide gel, and stained with 1
(from left, 62.5 ng/band, 20 ng/band, 10 ng/band, 5 ng/band, 1 ng/band and 0.5 ng/band)
Figure C. DNA molecular weight markers at each concentration were diluted, run on an acrylamide gel, and stained with 1
Figure C. DNA molecular weight markers at each concentration were diluted, run on an acrylamide gel, and stained with 1
(from left, 250 ng/lane, 50 ng/lane, 10 ng/lane, 2 ng/lane and 0.4 ng/lane)
 

References

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