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CAS RN: | Product Number: C4087
Chemiluminescence HRP Substrate Solution Kit [for Western Blotting]
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|---|---|---|---|---|---|
| 1KIT |
$155.00
|
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Supplemental Product Information:
"
Product Information
- Components : Chemiluminescence HRP Substrate Solution A 100 mL×1, Chemiluminescence HRP Substrate Solution B 100 mL×1
- Volume : 1 kit (for 2000 cm2 of membrane)
Description
- This product is a reagent used for the chemiluminescent detection of membrane-transferred HRP-labeled proteins in Western blotting. Immediately before use, solutions A and B are mixed 1:1 and applied to the membrane. Proteins can be detected down to nanogram level.
Storage
- The product should be stored at 4 °C and protected from light.
Direction for Use
-
- Bring Solution A and Solution B to room temperature.
- Prepare a western blot membrane labeled with an HRP-conjugated probe.
- Wash the membrane three times for 10 minutes each time with 1× TBS-T buffer.
- Mix Solution A and Solution B in a 1:1 ratio immediately before use. The recommended volume is 0.1 mL/cm2 membrane.
- Place the membrane protein side up on a sheet of the plastic wrap or polyethylene sheet.
- Gently pour the mixture evenly over the surface of the membrane.
- Incubate for 60 seconds at room temperature.
- Remove excess reagent by blotting with a tissue or tilting the membrane upright to allow excess to run off.
- 9. Place the membrane on a clean sheet of plastic wrap or polyethylene.
- Image the membrane using an imaging system or X-ray film.
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- W0024 Western Blot Stripping Buffer [for Biochemical Research]
| Product Number | C4087 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
Specifications
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H317 : May cause an allergic skin reaction. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P272 : Contaminated work clothing should not be allowed out of the workplace. P261 : Avoid breathing mist or vapors. P280 : Wear protective gloves. P302 + P352 : IF ON SKIN: Wash with plenty of water. P362 + P364 : Take off contaminated clothing and wash it before reuse. P333 + P313 : If skin irritation or rash occurs: Get medical advice/ attention. |
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Transport Information:
| H.S.code* | 3822.19-000 |
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