Product Document Searching Made Easy by 2D Code! | TCI Chemistry News October 2025 | [TCIPracticalExample] Di-Cbz Guanidinylation of Amine... | Various analytical charts can be searched on each product detail page and Product Document Search (The kinds of analytical charts differ by product)
Maximum quantity allowed is 999
Please select the quantity
CAS RN: | Product Number: Y0021
E.coli / Yeast Protein Extraction Buffer
×
Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Same Day | 2-3 Business Days | Other Lead Time |
Shipping Information
|
|---|---|---|---|---|---|
| 100ML |
$91.00
|
7 | 0 | Contact Us |
* Please contact our distributors or
TCI
to order our products. The above prices do not include freight cost, customs, and other charges to the destination.
* The storage conditions are subject to change without notice.
Supplemental Product Information:
Product Information
- Volume : 100 mL
- Additives : WITHOUT protease inhibitors
Description
- This product is supplied as a ready-to-use solution to lyse cultured E. coli / yeast cells. Extracted protein can be used in downstream applications such as Western blotting and protein quantification. This product does not include protease inhibitors.
Storage
- Store at 4 °C.
Directions for Use: Lysis of E. coli cells
-
- Centrifuge E. coli cells at 5000 × g for 10 minutes.
- Remove as much medium as possible from pellet.
- Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 5-10 mL buffer per gram pellet.
- Incubate for 10 minutes at room temperature.
- Centrifuge lysate at 15,000 × g for 10 minutes.
- Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Directions for Use: Lysis of yeast cells
-
- Centrifuge yeast cells at 3000 × g for 10 minutes.
- Remove as much medium as possible from pellet.
- Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
- Agitate at room temperature for 10 minutes.
- Centrifuge lysate at 14,000 × g for 10 minutes.
- Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Related Products
- A2215 AEBSF [for Biochemical Research]
- D3789 Disodium Dihydrogen Ethylenediaminetetraacetate Dihydrate [for Biochemical Research]
- E1337 E-64d [for Biochemical Research]
- P2575 Pyrogallol Red (Ready-to-use solution) [for Protein determination]
- P2949 100×Protease Inhibitor Cocktail (EDTA free)
- P2976 100×Protease Inhibitor Cocktail
| Product Number | Y0021 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Suitability for protein-analysis (E.coli) | to pass test |
| Suitability for protein-analysis (Yeast) | to pass test |
Properties (reference)
| Specific Gravity (20/20) | 1.06 |
GHS
Related Laws:
Transport Information:
| H.S.code* | 3822.19-000 |
Application
E.coli / Yeast Protein Extraction
References
- Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification
Product Articles
[Featured Products] Ready-to-use Solutions for Protein Extraction[TCIMAIL No.188] Ready-to-Use Solution for Protein Extraction from E. coli / Yeast Cells
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
Please select Language.
The requested SDS is not available.
Please Contact Us for more information.
Specifications
C of A & Other Certificates
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Sample C of A
This is a sample C of A and may not represent a recently manufactured lot of the product.
A sample C of A for this product is not available at this time.
Analytical Charts
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
The requested analytical chart is not available. Sorry for the inconvenience.