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Water-soluble Vitamins

Water-soluble Vitamins
Column Kaseisorb LC ODS 2000  4.6 mmI.D. × 150 mm
Mobile Phase CH3CN / 0.1% H3PO4 (pH 2.08)= 10 / 90
  containing 5mmol/L Sodium 1-Hexanesulfonate
Temperature 40 °C
Detection UV 210 nm
Flow Rate 1.0 mL/min
Sample 1.Nicotinic Acid 2.Calcium D-Pantothenate 3.Pyridoxine Hydrochloride 4.Folic Acid 5.Thiamine Hydrochloride 6.Riboflavin