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Fluorescent Probe: 5-CFDA

Carboxyfluorescein diacetate (CFDA) is used to measure intracellular pH.1) CFDA easily permeates cells and is converted to carboxyfluorescein. The carboxyfluorescein is retained better in cells compared with fluorescein. Therefore, CFDA is applied in the cell viability assay.2)
5-Carboxyfluorescein diacetate (5-CFDA, 1) is used for the FRAP assay by combination with another fluorescent probe. In the study on the heterocyst forming of cyanobacterium by Plominsky et al., 1 and calcein were used for the FRAP assay.3)
FRAP: fluorescence recovery after photobleaching
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