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N-Chloromethyl-4-nitrophthalimide [for HPLC Labeling]
(CAS RN:54455-37-7 Product Number:A5503)


Synonym 4-Nitro-N-chloromethylphthalimide

General Information

Product Number A5503

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Purity/Analysis Method >98.0%(T)
Storage Temperature
M.F. / M.W. C9H5ClN2O4=240.60
CAS RN 54455-37-7
Related CAS RN
MDL Number MFCD00059889
Packaging and Container
  • Product Details
  • Safety & Regulations
  • Reference & Application


Purity(Argentmetric Titration) min. 98.0 %
Melting point 153.0 to 157.0 deg-C
Solubility in hot Methanol almost transparency


Reaxys-RN 1474190
PubChem SID 87563153


Signal Word Warning
Hazard Statements
  • H315
  • :Causes skin irritation.
  • H319
  • :Causes serious eye irritation.
Precautionary Statements
  • P264
  • :Wash hands and face thoroughly after handling.
  • P280
  • :Wear protective gloves, eye protection.
  • P302+P352+P332+P313+P362+P364
  • :If on skin: Wash with plenty of soap and water. If skin irritation occurs: Get medical advice or attention. Take off contaminated clothing and wash it before reuse.
  • P305+P351+P338+P337+P313
  • :If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice or attention.


HPLC Labeling Reagent for Carboxylic Acids

The compound 1 is an HPLC labeling reagent, which has a chloromethyl group and easily reacts with a carboxyl group to form the corresponding ester in the presence of a base. The resultant ester is stable and can reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 230 nm.

Application example:
[Fatty acids]1, 2)
Dissolve 3 mg of a sample in 1 mL of acetonitrile, and add 1 mL of the labeling reagent 1 / acetonitrile solution (11 mg / mL) and 1 mL of triethylamine / acetonitrile solution (5 mg / mL). Close the cap of the reaction vessel and incubate the solution at 60 ºC for 1 h. Cool the resultant solution to room temperature and use it as an HPLC sample. In the case of using alkali metal salts and crown ethers, the esterification reaction is completed in 15 min at 60 ºC. Cool the resultant solution to room temperature and use it as an HPLC sample.


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