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O-4-Nitrobenzylhydroxylamine Hydrochloride [for HPLC Labeling]
(CAS RN:2086-26-2 Product Number:A5532)


O-4-Nitrobenzylhydroxylamine Hydrochloride

General Information

Product Number A5532

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Purity/Analysis Method >99.0%(HPLC)
Storage Temperature
M.F. / M.W. C7H8N2O3·HCl=204.61
CAS RN 2086-26-2
Related CAS RN
MDL Number MFCD00012954
Packaging and Container
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Appearance White to Light yellow powder to crystal
Purity(HPLC) min. 99.0 area%
Purity(Argentmetric Titration) min. 98.0 %
Solubility in hot Water almost transparency
Effect test of HPLC derivatization Effective as labeling agent for Valeraldehyde


Beilstein 6(2)426
Reaxys-RN 3709565
PubChem SID 87563167
RTECS# NC4400050
EC Number 218-228-6

Transport Information

HS Number 2921490090


HPLC Labeling Reagent for Carbonyl Compounds

The compound 1 is an HPLC labeling reagent, which has a hydroxylamino moiety, can easily react with a carbonyl group to form the corresponding oxime. The resultant oxime is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 254 nm, an absorption wavelength that is generally used for UV detection.

Application examples:
[Aldehydes] 1)
1~5 mg of a sample, 4 mL of methanol, 2 drops of triethylamine, and 40 mg of the labeling reagent 1 are mixed. Close the cap of the reaction vessel and incubate the mixture at 65 ºC for 1 h. After cooling to room temperature, use it as the HPLC sample solution. If it is necessary to remove the unreacted labeling reagent and triethylamine, evaporate the solvent at a low temperature under a nitrogen atmosphere. Dissolve the residue in 2~3 mL of ether and wash with diluted HCl and water.


  • 1)T. H. Jupille, Am. Lab. 1976, 8, 85.

PubMed Literature

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