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Streptavidin Maleimide Conjugate
(Product Number:T3531)

Structure

Streptavidin Maleimide Conjugate

General Information

Product Number T3531

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Purity/Analysis Method
Storage Temperature -80°C
M.F. / M.W.
CAS RN
Related CAS RN
MDL Number
Packaging and Container

Notes

Product Information
Volume/Quantity : 0.5 mg
Conjugation : Purified streptavidin was conjugated to Sulfo-SMCC
(TCI : code S0883).
Product Form : Streptavidin Maleimide Conjugate.
Lyophilized in 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.2.
Preservative : None
Specific Activity : >10 U/mg , HABA dye-binding method(1)

Description
Streptavidin Maleimide Conjugate is designed to facilitate the conjugation of the Streptavidin to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups.

Protocol
1. Allow the vial of Streptavidin Maleimide Conjugate to warm to room temperature.
2. Reconstitute the Streptavidin Maleimide Conjugate by adding a buffered solution of the thiol-containing molecule. Gently agitate the vial to ensure thorough dissolution.
Note: The buffer in which the thiol-containing molecule is dissolved should be in the pH range 6.5-7.5. Phosphate buffered saline pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA (1-10 mM) may help to prevent metal catalysed oxidation of thiols.
3. React for 30–60 minutes at 37 °C or 2 hours at room temperature.
4. If desired, desalt, dialyze or purify the conjugate.

Storage
Store at -20 °C to -80 ºC (Preferably at -80 ºC). Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.

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Specification

Specific Activity to pass test

References

PubChem SID 354335029

Transport Information

HS Number 3504009000

Application

Streptavidin Maleimide Conjugate

References

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