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2-(4-Amidinophenyl)indole-6-carboxamidine Dihydrochloride [for Biochemical Research]
(CAS RN:28718-90-3 Product Number:A2412)

Structure

2-(4-Amidinophenyl)indole-6-carboxamidine Dihydrochloride
Synonym 2-(4-Carbamimidoylphenyl)indole-6-carboximidamide Dihydrochloride
Synonym DAPI·2HCl
Synonym 4',6-Diamidino-2-phenylindole Dihydrochloride

General Information

Product Number A2412

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Purity/Analysis Method >90.0%(HPLC)
Storage Temperature <0°C
M.F. / M.W. C16H15N5·2HCl=350.25
CAS RN 28718-90-3
Related CAS RN
MDL Number MFCD00012681
Packaging and Container
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Specification

Appearance Light yellow to Yellow to Orange powder to crystal
Purity(HPLC) min. 90.0 area%
DNA staining test to pass test

References

Reaxys-RN 4894417
PubChem SID 135727109
EC Number 249-186-7

Transport Information

HS Number 2933998090

Application

Fluorescence Staining of Cell Nucleus

DAPI is an organic dye which binds strongly to DNA. DAPI emits a blue fluorescence (Maximum fluorescent wavelength; 461 nm) by the irradiation of UV light (Maximum excitation wavelength; 358 nm).
HeLa cell nucleus stained with DAPI·2HCl [A2412] has been observed by fluorescent microscopy.

A2412

HeLa cells were fixed with 4% paraformaldehyde, and permerabilized with 0.2% Triton X-100.
HeLa cells nuclei were stained with 1 μg/mL DAPI [A2412] solution.

References

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