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2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride Hydrate [for Biochemical Research]
(CAS RN:23491-45-4 Product Number:H1343)


2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride
Synonym Bisbenzimide H 33258 Hydrate

General Information

Product Number H1343

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Purity/Analysis Method >98.0%(HPLC)
Storage Temperature <0°C
M.F. / M.W. C25H24N6O·3HCl·xH2O=533.88(as Anhydrous)
CAS RN 23491-45-4
Related CAS RN
MDL Number MFCD00012679
Packaging and Container
  • Product Details
  • Safety & Regulations


Appearance Light yellow to Brown to Dark green powder to crystal
Purity(HPLC) min. 98.0 area%
Water(Vaporization methoed) max. 20.0 %
DNA staining test to pass test

Data of Reference

mp 280°C(lit.)


Reaxys-RN 4088183
PubChem SID 172089156
RTECS# SM1140500
EC Number 245-690-6


Signal Word Warning
Hazard Statements
  • H315
  • :Causes skin irritation.
  • H319
  • :Causes serious eye irritation.
Precautionary Statements
  • P264
  • :Wash hands and face thoroughly after handling.
  • P280
  • :Wear protective gloves, eye protection.
  • P302+P352+P332+P313+P362+P364
  • :IF ON SKIN: Wash with plenty of water. If skin irritation occurs: Get medical advice or attention. Take off contaminated clothing and wash it before reuse.
  • P305+P351+P338+P337+P313
  • :IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice or attention.

Transport Information

HS Number 3212900000


Immunofluorescence Staining

The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.
*AIF normally localize in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] at 5 µg/mL (shown in red)
  Goat Anti-Rabbit IgG FITC Conjugate [G0452] at 5 µg/mL (shown in green)
  Hoechst 33258 [H1343] at 1 µg/mL (shown in blue)

Fluorometric Assays of DNA Using Bisbenzimide H 33258


PubMed Literature

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