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Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]
(Product Number:N1113)


Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution
Synonym NBT / X-Phosphate p-Toluidine Salt Solution (50X)

General Information

Product Number N1113

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Purity/Analysis Method
Storage Temperature 0-10°C
M.F. / M.W.
Related CAS RN
MDL Number
Packaging and Container


Product Information
Volume : 5 mL

This product is supplied as a concentrated solution containing Nitro blue tetrazolium (NBT) and 5-Bromo-4-chloro-3-indolyl phosphate (X-Phosphate p-Toluidine Salt) for Western Blotting. When this product is reacted with alkaline phosphatase (ALP), a blue-purple precipitate appears.

Direction for Use
1: Incubate a blotting membrane with an ALP-conjugated antibody and wash the membrane.
2: Dilute NBT/ X-Phosphate p-Toluidine Salt solution (Product No. N1113) to 1X before use.
(Dilute solution : 20 mM Tris-HCl pH 9.5, 2mM MgCl2 and 100 mM NaCl)
3: Incubate the washed membrane with 1X NBT/ X-Phosphate p-Toluidine Salt solution until color development.
4: Add deionized water to stop the color development.

The product should be stored at 4 ºC. Don’t freeze because of arising precipitate.

Related Products
B1239 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt [for Biochemical Research]
D0844 Nitro blue tetrazolium [for Biochemical Research]
C3384 4-Chloro-1-naphthol (Ready-to-use solution) [for Western blotting]
N1109 4-Nitrophenyl Phosphate (Ready-to-use solution) [for ELISA]
  • Product Details
  • Safety & Regulations


Appearance Yellow to Amber to Dark purple clear liquid
Alkaline Phosphatase detection test to pass test


Signal Word Warning
Hazard Statements
  • H373
  • :May cause damage to Liver, Blood through prolonged or repeated exposure.
Precautionary Statements
  • P260
  • :Do not breathe mist, vapors or spray.
  • P314
  • :Get medical advice or attention if you feel unwell.
  • P501
  • :Dispose of contents and container in accordance with local, regional, national regulations (e.g. US: 40 CFR Part 261, EU:91/156/EEC, JP: Waste Disposal and Cleaning Act, etc.).


Nitro Blue Tetrazolium / 5-Bromo-4-chloro-3-indolyl Phosphate p-Toluidine Salt Solution (50X) [for Western blotting]


  • Cold Spring Harbor Laboratory Press, New York
    • E. Harlow and D. Lane., Antibodies, A Laboratory Manual, 1988, p407, p505, p598

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