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NBD-PZ (=4-Nitro-7-piperazino-2,1,3-benzoxadiazole) [for HPLC Labeling]
(CAS RN:139332-66-4 Product Number:A5554)

Structure

NBD-PZ
Synonym 4-Nitro-7-piperazinobenzofurazan
Synonym 4-Nitro-7-piperazino-2,1,3-benzoxadiazole

General Information

Product Number A5554

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Purity/Analysis Method >98.0%(HPLC)(T)
Storage Temperature
M.F. / M.W. C10H11N5O3=249.23
CAS RN 139332-66-4
Related CAS RN
MDL Number MFCD00191507
Packaging and Container
  • Product Details
  • Safety & Regulations

Specification

Purity(HPLC) min. 98.0 area%
Purity(Nonaqueous Titration) min. 98.0 %
Effect test of HPLC derivatization Effective as labeling agent for Linoleic Acid , Oleic Acid

References

Reaxys-RN 7936339
PubChem SID 87563172

GHS

Pictogram
Signal Word Warning
Hazard Statements
  • H315
  • :Causes skin irritation.
  • H319
  • :Causes serious eye irritation.
Precautionary Statements
  • P264
  • :Wash hands and face thoroughly after handling.
  • P280
  • :Wear protective gloves, eye protection.
  • P302+P352+P332+P313+P362+P364
  • :IF ON SKIN: Wash with plenty of water. If skin irritation occurs: Get medical advice or attention. Take off contaminated clothing and wash it before reuse.
  • P305+P351+P338+P337+P313
  • :IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice or attention.

Transport Information

HS Number 2934999090

Application

HPLC Labeling Reagent for Carboxylic Acids

The compound 1 is an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a piperazino group, easily reacts with a carboxyl group at room temperature to form the corresponding amide in the presence of a condensation reagent. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 470 nm and 541 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference by contaminants. A highly sensitive detection can be done by using laser induced fluorescence detector.

Application examples:
[Fatty acids] 1)
0.2 mL of 140 mM DEPC or 70 mM 2,2’-dipyridyl disulfide-Ph3P / DMF solution containing a fatty acid (10 μM) is added to 0.2 mL of the labeling reagent 1 / DMF or acetonitrile solution (10 mM). React at room temperature for 6 h, then use it as an HPLC sample.

References

PubMed Literature

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