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NBD-H (=4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine) [for HPLC Labeling]
(CAS RN:131467-87-3 Product Number:A5557)


Synonym 4-Hydrazino-7-nitrobenzofurazan Hydrazine
Synonym 4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine

General Information

Product Number A5557

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Purity/Analysis Method >98.0%(HPLC)
Storage Temperature 0-10°C
M.F. / M.W. C6H5N5O3·H4N2=227.18
CAS RN 131467-87-3
Related CAS RN
MDL Number MFCD00191508
Packaging and Container
  • Product Details
  • Safety & Regulations


Purity(HPLC) min. 98.0 area%
Effect test of HPLC derivatization Effective as labeling agent for Propionaldehyde


PubChem SID 87563175


Signal Word Warning
Hazard Statements
  • H315
  • :Causes skin irritation.
  • H319
  • :Causes serious eye irritation.
Precautionary Statements
  • P264
  • :Wash hands and face thoroughly after handling.
  • P280
  • :Wear protective gloves, eye protection.
  • P302+P352+P332+P313+P362+P364
  • :IF ON SKIN: Wash with plenty of water. If skin irritation occurs: Get medical advice or attention. Take off contaminated clothing and wash it before reuse.
  • P305+P351+P338+P337+P313
  • :IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice or attention.

Transport Information

HS Number 2934999090


HPLC Labeling Reagent for Carbonyl Compounds

The compound 1 is an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a hydrazino group, easily reacts with a carbonyl group to form the corresponding hydrazone. The labeling reagent itself is non-fluorescent, but the hydrazones after the reaction with carbonyl compounds have strong fluorescence. The resultant hydrazone is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 470 nm and 550 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference by contaminants, and a highly sensitive detection can be done because of its high reactivity.

Application examples:
[Aldehydes or ketones] 1)
250 μM of the labeling reagent 1 and 1.7 μM propionaldehyde are added to acetonitrile containing 0.025% TFA, and reacted at room temperature for 1 h, use it as the HPLC sample.
For example, the detection limit for propionaldehyde is 35 fmol.


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