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DBD-COCl [=4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
(CAS RN:156153-43-4 Product Number:A5558)

Structure

DBD-COCl
Synonym 4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)benzofurazan
Synonym 4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole

General Information

Product Number A5558

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Purity/Analysis Method >93.0%(T)
Storage Temperature <0°C
M.F. / M.W. C11H13ClN4O4S=332.76
CAS RN 156153-43-4
Related CAS RN
MDL Number MFCD00191377
Packaging and Container
  • Product Details
  • Safety & Regulations

Specification

Purity(Argentmetric Titration) min. 93.0 %
Effect test of HPLC derivatization Effective as labeling agent for Benzylamine

References

PubChem SID 87563176

GHS

Pictogram
Signal Word Danger
Hazard Statements
  • H290
  • :May be corrosive to metals.
  • H314
  • :Causes severe skin burns and eye damage.
Precautionary Statements
  • P260
  • :Do not breathe dusts or mists.
  • P280
  • :Wear protective gloves, protective clothing, face protection.
  • P301+P330+P331+P310
  • :IF SWALLOWED: Rinse mouth. Do NOT induce vomiting. Immediately call a POISON CENTER or doctor.
  • P303+P361+P353+P310+P363
  • :IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water or shower. Immediately call a POISON CENTER or doctor. Wash contaminated clothing before reuse.
  • P304+P340+P310
  • :IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER or doctor.
  • P305+P351+P338+P310
  • :IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor.

Transport Information

UN Number 3261
Class 8
Packing Group III
HS Number 2935909099

Application

HPLC Labeling Reagent

The compound 1 is an HPLC fluorescence labeling reagent, which reacts with many kinds of nucleophilic groups under mild conditions. The reaction examples are shown in the table below.
These resulting compounds are stable, and can reach the detector without any decomposition under reversed phase HPLC, thus excellent chromatograms can be obtained by fluorescence detection.
Groups Examples Reaction Conditions Wavelengths (nm) Detection Limits (fmol)
ex em
Alcohols Androsterone 60℃, 30 min 443 546 38
α-Oxyacids Mandelic acid 60℃, 15 min 442 551 125
Phenols Estrone 60℃, 15 min 440 543 40
Amines Benzylamine r.t. or 60℃, 15 min 445 555 89
Aromatic amines Phenetidine 60℃, 15 min 443 553 56
Thiols 2-Mercapto-N-(2-naphthyl)acetamide r.t. 437 544 103

Application examples:
10 μL of 25 mM labeling reagent 1 in dry benzene is mixed with 10 μL of 0.5 mM androsterone in dry benzene (containing 0.5 mM quinuclidine*), and incubated at 60 ºC for 30 min. The reaction solution is quenched with 980 μL of 50% acetonitrile solution containing 1% acetic acid, use it as the HPLC sample solution.
*For primary alcohols, quinuclidine is not necessarily needed.

References

PubMed Literature

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