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NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
(CAS RN:221263-97-4 Product Number:A5573)

Structure

NBD-CO-Hz
Synonym 4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitrobenzofurazan
Synonym 4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole

General Information

Product Number A5573

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Purity/Analysis Method >95.0%(HPLC)
Storage Temperature 0-10°C
M.F. / M.W. C9H10N6O4=266.22
CAS RN 221263-97-4
Related CAS RN
MDL Number MFCD02093433
Packaging and Container
  • Product Details
  • Safety & Regulations

Specification

Purity(HPLC) min. 95.0 area%
Elemental analysis(Nitrogen) 30.00 to 33.15 %
Effect test of HPLC derivatization Effective as labeling agent fo Ibuprofen

References

PubChem SID 87563190

GHS

Pictogram
Signal Word Warning
Hazard Statements
  • H315
  • :Causes skin irritation.
  • H319
  • :Causes serious eye irritation.
Precautionary Statements
  • P264
  • :Wash hands and face thoroughly after handling.
  • P280
  • :Wear protective gloves, eye protection.
  • P302+P352+P332+P313+P362+P364
  • :IF ON SKIN: Wash with plenty of water. If skin irritation occurs: Get medical advice or attention. Take off contaminated clothing and wash it before reuse.
  • P305+P351+P338+P337+P313
  • :IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice or attention.

Transport Information

HS Number 2934999090

Application

HPLC Labeling Reagent for Carboxylic Acids

The compound 1 an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a hydrazino group, easily reacts with a carboxyl group to form the corresponding carbohydrazide in the presence of a condensing agent. The resultant carbohydrazide is stable for at least one week at 4 ºC. The carbohydrazide derivatives can be analyzed by reversed phase HPLC, and an excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 475 nm and 530 nm, respectively. [The detection limit = 2~4 fmol (S/N = 3)]

Application example:
Add 50 μL of carboxylic acid / DMF solution, 50 μL of 1.0 M EDC aqueous solution, 50 μL of 20% pyridine aqueous solution and 20 mM HPLC labeling reagent 1 / DMF solution to a vessel, and incubate the mixture at room temperature for 2 h. Dilute this reactant mixture 10x with the mobile phase solution, and use 1 μL of this diluted solution as an HPLC sample solution.

References

PubMed Literature

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