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DBD-ED [=4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
(CAS RN:189373-41-9 Product Number:A5574)


Synonym 4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole

General Information

Product Number A5574

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Purity/Analysis Method
Storage Temperature
M.F. / M.W. C10H15N5O3S=285.32
CAS RN 189373-41-9
Related CAS RN
MDL Number MFCD01321129
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Elemental analysis(Nitrogen) 23.30 to 25.80 %
Effect test of HPLC derivatization Effective as labeling agent for Linoleic Acid , Oleic Acid


Reaxys-RN 18820975
PubChem SID 87563191

Transport Information

HS Number 2935909099


HPLC Labeling Reagent for Carboxylic Acids

The compound 1, an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and an amino group, easily reacts with a carboxyl group to form the corresponding amide in the presence of a condensing agent. The resultant amide is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 450 nm and 560 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference from contaminants. Short-chain fatty acids are detectable and determinable reproducibly with a detection limit on the order of fmol. A highly sensitive detection can be done by using chemiluminescence.

Application example:2)
Add 50 μL of mixed fatty acid / diethyl ether solution, 50 μL of 50 mM labeling reagent 1 / acetonitrile solution, 50 μL of triphenylphosphine / acetonitrile solution and 50 μL of 2,2’-dipyridyl disulfide / acetonitrile solution to a vessel. This mixture is kept in the dark at room temperature. Dilute this reactant mixture 100x by acetonitrile, and use 10 μL of this diluted solution as an HPLC sample solution.


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