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AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]
(CAS RN:254973-02-9 Product Number:A5576)

Structure

AABD-SH
Synonym 4-Acetamido-7-mercaptobenzofurazan
Synonym 4-Acetamido-7-mercapto-2,1,3-benzoxadiazole

General Information

Product Number A5576

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Purity/Analysis Method >95.0%(HPLC)
Storage Temperature 0-10°C
M.F. / M.W. C8H7N3O2S=209.22
CAS RN 254973-02-9
Related CAS RN
MDL Number MFCD04038408
Packaging and Container
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Specification

Purity(HPLC) min. 95.0 area%
Effect test of HPLC derivatization Effective as labeling agent for Caproic Acid, Lauric Acid, Myristic Acid, Palmitic Acid

References

PubChem SID 87563193

Transport Information

HS Number 2934999090

Application

HPLC Labeling Reagent for Carboxylic Acids

The compound 1, an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a mercapto group, easily reacts with a carboxyl group to form the corresponding thioester. 1 itself fluoresces very little, but the thioester derivatives fluoresce highly. The resultant thioester is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 368 nm and 524 nm, respectively. [The detection limit = 10~20 fmol (S/N = 3)]

Application example:
Add 20 μL of mixed fatty acid / acetonitrile solution, 20 μL of 20 mM labeling reagent 1 / dichloromethane solution, 20 μL of triphenylphosphine / acetonitrile solution and 20 μL of 2,2’-dipyridyl disulfide / acetonitrile solution to a 500 μL vessel, and the mixture is left at room temperature for 15 min. Dilute this reactant mixture with 20 μL of acetonitrile, and use 1 μL of this diluted solution as an HPLC sample solution.

References

PubMed Literature

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