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NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
(CAS RN:10199-89-0 Product Number:A5592)


Synonym 4-Chloro-7-nitrobenzofurazan
Synonym 4-Chloro-7-nitro-2,1,3-benzoxadiazole

General Information

Product Number A5592

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Purity/Analysis Method >98.0%(T)
Storage Temperature
M.F. / M.W. C6H2ClN3O3=199.55
CAS RN 10199-89-0
Related CAS RN
MDL Number MFCD00005808
Packaging and Container
  • Product Details
  • Safety & Regulations


Purity(Butylamine Method) min. 98.0 %
Melting point 97.0 to 100.0 deg-C
Solubility in Methanol almost transparency
Effect test of HPLC derivatization Effective as labeling agent for Butylamine , Propylamine


Reaxys-RN 614212
PubChem SID 87563199
RTECS# DF8002400
EC Number 233-496-4


Signal Word Warning
Hazard Statements
  • H315
  • :Causes skin irritation.
  • H319
  • :Causes serious eye irritation.
Precautionary Statements
  • P264
  • :Wash hands and face thoroughly after handling.
  • P280
  • :Wear protective gloves, eye protection.
  • P302+P352+P332+P313+P362+P364
  • :IF ON SKIN: Wash with plenty of water. If skin irritation occurs: Get medical advice or attention. Take off contaminated clothing and wash it before reuse.
  • P305+P351+P338+P337+P313
  • :IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice or attention.

Transport Information

HS Number 2934999090


HPLC Labeling Reagent for Amines and Thiols

The compound 1 which is an HPLC fluorescence labeling reagent having a 2,1,3-benzoxadiazole skeleton, can easily react with a secondary amine and thiol. The resultant derivative is stable enough to reach the detector without any decomposition under general reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection analysis at the excitation and emission wavelengths of 460 nm and 535 nm, respectively.

Application example:
[Alkylamines] 1)
To 25~500 μL of a methanol solution containing an amine (1~20 μg), 4~8 eq. excess amount of 0.05% labeling reagent 1 / methanol solution is added. After adding 50~100 μL of 0.1 M NaHCO3, incubate at 55 ºC for 1~5 h. After cooling the reaction mixture to room temperature, use it as an HPLC sample.
TLC and HPLC of N-methylcarbamates, N,N-dimethylcarbamates in agrichemicals2,3) Hydrolyze the carbamates to label the amine derivatives.
TLC of amphetamines in urine4,5), HPLC of prolines (precolumn derivatization method)6)


PubMed Literature

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