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NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
(CAS RN:29270-56-2 Product Number:A5593)


Synonym 4-Fluoro-7-nitrobenzofurazan
Synonym 4-Fluoro-7-nitro-2,1,3-benzoxadiazole

General Information

Product Number A5593

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Purity/Analysis Method >99.0%(HPLC)
Storage Temperature <0°C
M.F. / M.W. C6H2FN3O3=183.10
CAS RN 29270-56-2
Related CAS RN
MDL Number MFCD00010196
Packaging and Container
  • Product Details
  • Safety & Regulations


Purity(HPLC) min. 99.0 area%
Melting point 53.0 to 57.0 deg-C
Solubility in Chloroform very faint turbidity
Effect test of HPLC derivatization Effective as labeling agent for L-Isoleucine


Reaxys-RN 1077571
PubChem SID 87563200


Signal Word Warning
Hazard Statements
  • H315
  • :Causes skin irritation.
  • H319
  • :Causes serious eye irritation.
Precautionary Statements
  • P264
  • :Wash hands and face thoroughly after handling.
  • P280
  • :Wear protective gloves, eye protection.
  • P302+P352+P332+P313+P362+P364
  • :IF ON SKIN: Wash with plenty of water. If skin irritation occurs: Get medical advice or attention. Take off contaminated clothing and wash it before reuse.
  • P305+P351+P338+P337+P313
  • :IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice or attention.

Transport Information

HS Number 2934999090


HPLC Labeling Reagent for Amines and Thiols

The compound 1 which is an HPLC fluorescence labeling reagent having a 2,1,3-benzoxadiazole skeleton, can easily react with amino or mercapto groups to form the corresponding derivatives. 1 itself does not fluoresce, and its ethanol solution is relatively stable for a week in a refrigerator. The derivatives can reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 470 nm and 530 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference by contaminants. Thus, further highly sensitive detection can be done by using laser induced fluorescence detector. When the reagent is hydrolyzed (NBD-OH), its fluorescence can be erased under an acidic condition. Therefore, this hydrolyzed reagent can be used as a post column reaction reagent5,7).

Application example:
[Amino acids] 2,3)
To 10 μL of 50 μM amino acid standard solution, add 10 μL of 0.1 M boric acid buffer solution (pH 8.0) and 20 μL of 50 mM labeling reagent 1 in ethanol solution, and incubate the mixture at 60 ºC for 1 min. Immediately cool it with ice bath, and add 460 μL of 5 mM HCl to the reactant solution. Use 10 μL of the solution as an HPLC sample.


PubMed Literature

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