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Methotrexate Hydrate
(CAS RN:133073-73-1 Product Number:M1664)


Synonym (+)-Amethopterin Hydrate

General Information

Product Number M1664

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Purity/Analysis Method >98.0%(HPLC)(T)
Storage Temperature
M.F. / M.W. C20H22N8O5·xH2O=454.45(as Anhydrous)
CAS RN 133073-73-1
Related CAS RN 59-05-2
MDL Number MFCD00150847
Packaging and Container
  • Product Details
  • Safety & Regulations


Purity(HPLC) min. 98.0 area%
Purity(Neutralization titration) min. 98.0 %(calcd.on anh.substance)
Specific rotation [a]20/D +18.0 to +23.0 deg(C=0.1, 1mol/L NaOH sol.)(calcd.on anh.substance)
Water max. 10.0 %


Beilstein 26(3/4)3833
Reaxys-RN 70669
PubChem SID 87558856
Merck Index(14) 5985
RTECS# MA1225000
EC Number 200-413-8


Signal Word Danger
Hazard Statements
  • H301
  • :Toxic if swallowed.
  • H315
  • :Causes skin irritation.
  • H319
  • :Causes serious eye irritation.
  • H360FD
  • :May damage fertility. May damage the unborn child.
  • H372
  • :Causes damage to Liver, Blood, Bone Marrow, Bone, Kidney, Lung through prolonged or repeated exposure.
Precautionary Statements
  • P260
  • :Do not breathe dust, fume, mist, vapours or spray.
  • P280
  • :Wear protective gloves, protective clothing, face protection.
  • P301+P310+P330
  • :IF SWALLOWED: Immediately call a POISON CENTER or doctor. Rinse mouth.
  • P302+P352+P332+P313+P362+P364
  • :IF ON SKIN: Wash with plenty of water. If skin irritation occurs: Get medical advice or attention. Take off contaminated clothing and wash it before reuse.
  • P305+P351+P338+P337+P313
  • :IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice or attention.
  • P308+P313
  • :IF exposed or concerned: Get medical advice or attention.

Transport Information

UN Number 2811
Class 6.1
Packing Group III
HS Number 2933998090


Methotrexate: An Inhibitor of Dihydrofolate Reductase (DHFR)

Methotrexate (MTX), analog of folic acid, is an inhibitor of the enzyme dihydrofolate reductase (DHFR) which catalyzes the conversion of dihydrofolate to the active tetrahydrofolate, leading to interfere with folic acid metabolism. Result is inhibition of DNA synthesis and cell proliferation in S phases (the phase of DNA synthesis). Also, MTX has immunosuppressive and anti-inflammatory activity. Although no single mechanism is sufficient to account for all the anti-inflammatory activities of methotrexate, the release of adenosine from cells has been demonstrated. MTX is commonly used for the treatment of certain cancers including leukemia, rheumatoid arthritis, Crohn’s disease and psoriasis. (The product is for research purpose only.)


PubMed Literature

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